PDE6G sirna

PDE6G siRNA (Mouse)

Cat. Num. AAA8205730

• Gene Names: Pde6g; Pdeg; p3AT
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PDE6G; PDE6G siRNA (Mouse); PDEG; Retinal rod rhodopsin-sensitive cGMP 3'.5'-cyclic phosphodiesterase subunit gamma; GMP-PDE gamma; PDE6G sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PDE6G siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 87

• Applicable Applications for PDE6G sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PDE6G gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PDE6G sirna

siRNA to inhibit PDE6G expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7549783
• NCBI GeneID: 18588
• NCBI Accession #: NP_036197.1
• NCBI GenBank Nucleotide #: NM_012065.3
• UniProt Accession #: P09174
• Molecular Weight: 9,637 Da
• NCBI Official Full Name: retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma
• NCBI Official Synonym Full Names: phosphodiesterase 6G, cGMP-specific, rod, gamma
• NCBI Official Symbol: Pde6g
• NCBI Official Synonym Symbols: Pdeg; p3AT
• NCBI Protein Information: retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma
• UniProt Protein Name: Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma
• Protein Family: Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: Pde6g
• UniProt Synonym Gene Names: Pdeg; GMP-PDE gamma
• UniProt Entry Name: CNRG_MOUSE

Uniprot Description

PDE6G: inhibitory gamma subunit of the retinal photoreceptor type 6 cGMP phosphodiesterase (PDE-gamma). Phosphorylated by GRK2. Regulates EGFR dependent stimulation of ERK1/2. Forms a cellular complex with src. cGMP-PDEs are the effector molecules in G-protein-mediated phototransduction in vertebrate rods and cones. Participates in processes of transmission and amplification of the visual signal.

Protein type: Nucleotide Metabolism - purine; EC 3.1.4.35; Phosphodiesterase

Molecular Function: 3',5'-cyclic-GMP phosphodiesterase activity; protein binding; spectrin binding; hydrolase activity; cGMP binding; 3',5'-cyclic-nucleotide phosphodiesterase activity

Biological Process: visual perception; activation of MAPK activity; positive regulation of G-protein coupled receptor protein signaling pathway; positive regulation of epidermal growth factor receptor signaling pathway; response to stimulus