OAZ2 sirna

OAZ2 siRNA (Mouse)

Cat. Num. AAA8201951

• Gene Names: Oaz2; AZ2; AZ-2; Sez15; Oaz2-ps
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: OAZ2; OAZ2 siRNA (Mouse); SEZ15; Ornithine decarboxylase antizyme 2; AZ2; ODC-Az 2; Seizure-related protein 15; OAZ2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: OAZ2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 189

• Applicable Applications for OAZ2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse OAZ2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for OAZ2 sirna

siRNA to inhibit OAZ2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 11136624
• NCBI GeneID: 18247
• NCBI Accession #: NP_035082.1
• NCBI GenBank Nucleotide #: NM_010952.3
• UniProt Accession #: O08608
• Molecular Weight: 21,025 Da
• NCBI Official Full Name: ornithine decarboxylase antizyme 2 isoform 1
• NCBI Official Synonym Full Names: ornithine decarboxylase antizyme 2
• NCBI Official Symbol: Oaz2
• NCBI Official Synonym Symbols: AZ2; AZ-2; Sez15; Oaz2-ps
• NCBI Protein Information: ornithine decarboxylase antizyme 2
• UniProt Protein Name: Ornithine decarboxylase antizyme 2
• Protein Family: Ornithine decarboxylase antizyme
• UniProt Gene Name: Oaz2
• UniProt Synonym Gene Names: Sez15; AZ2; ODC-Az 2
• UniProt Entry Name: OAZ2_MOUSE

NCBI Description

The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamines. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis; thus, completing the auto-regulatory circuit. This gene encodes antizyme 2, the second member of the antizyme family. Like antizyme 1, antizyme 2 has broad tissue distribution, inhibits ODC activity and polyamine uptake, and stimulates ODC degradation in vivo; however, it fails to promote ODC degradation in vitro. Antizyme 2 is expressed at lower levels than antizyme 1, but is evolutionary more conserved, suggesting it likely has an important biological role. Studies also show different subcellular localization of antizymes 1 and 2, indicating specific function for each antizyme in discrete compartments of the cell. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2014]

Uniprot Description

OAZ2: Binds to, and destabilizes, ornithine decarboxylase. Does not accelerate ornithine decarboxylase degeneration. Belongs to the ODC antizyme family. 1 isoforms of the human protein are produced by ribosomal frameshifting.

Protein type: Inhibitor

Cellular Component: nucleus; cytosol

Molecular Function: enzyme inhibitor activity; protein binding; ornithine decarboxylase inhibitor activity

Biological Process: polyamine biosynthetic process; negative regulation of catalytic activity; positive regulation of protein catabolic process; polyamine metabolic process