Rabbit anti-Human TFF1 Polyclonal Antibody | anti-TFF1 antibody

TFF1 Antibody (Center)

Cat. Num. AAA9217231

• Gene Names: TFF1; pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
• Reactivity: Human
• Applications: Western Blot, ELISA
• Synonyms: TFF1; Polyclonal Antibody; TFF1 Antibody (Center); Trefoil factor 1; Breast cancer estrogen-inducible protein; PNR-2; Polypeptide P1.A; hP1.A; Protein pS2; BCEI; PS2; anti-TFF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This TFF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 11-40 amino acids from the Central region of human TFF1.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 84

• Applicable Applications for anti-TFF1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB:~~1:2000
• Subtitle: Purified Rabbit Polyclonal Antibody (Pab)
• Cellular Location: Secreted.
• Tissue Location: Found in stomach, with highest levels in the upper gastric mucosal cells (at protein level). Detected in goblet cells of the small and large intestine and rectum, small submucosal glands in the esophagus, mucous acini of the sublingual gland, submucosal glands of the trachea, and epithelial cells lining the exocrine pancreatic ducts but not in the remainder of the pancreas (at protein level). Scattered expression is detected in the epithelial cells of the gallbladder and submucosal glands of the vagina, and weak expression is observed in the bronchial goblet cells of the pseudostratified epithelia in the respiratory system (at protein level). Detected in urine (at protein level) Strongly expressed in breast cancer but at low levels in normal mammary tissue. It is regulated by estrogen in MCF-7 cells. Strong expression found in normal gastric mucosa and in the regenerative tissues surrounding ulcerous lesions of gastrointestinal tract, but lower expression found in gastric cancer (at protein level).
• Antigen Type: Synthesized Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-TFF1 Antibody (Center) at 1:2000 dilutionLane 1: Jurkat whole cell lysateLane 2: T47D whole cell lysateLane 3: SK-BR-3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 9 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-TFF1 antibody

Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.

Product Categories/Family for anti-TFF1 antibody

Crown Antibodies; Primary Antibodies; Signal Transduction

References

Jakowlew S.B.,et al.Nucleic Acids Res. 12:2861-2878 (1984). Prud'Homme J.-F.,et al.DNA 4:11-21 (1985). Jeltsch J.-M.,et al.Nucleic Acids Res. 15:1401-1414 (1987). Takahashi H.,et al.FEBS Lett. 261:283-286 (1990). Mori K.,et al.J. Biochem. 107:73-76 (1990).

NCBI and Uniprot Product Information

• NCBI GI #: 4507451
• NCBI GeneID: 7031
• NCBI Accession #: NP_003216.1
• NCBI GenBank Nucleotide #: NM_003225.2
• UniProt Accession #: P04155
• Molecular Weight: 9150 Da
• NCBI Official Full Name: trefoil factor 1
• NCBI Official Synonym Full Names: trefoil factor 1
• NCBI Official Symbol: TFF1
• NCBI Official Synonym Symbols: pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
• NCBI Protein Information: trefoil factor 1
• UniProt Protein Name: Trefoil factor 1
• Protein Family: Trefoil factor
• UniProt Gene Name: TFF1
• UniProt Synonym Gene Names: BCEI; PS2; hP1.A
• UniProt Entry Name: TFF1_HUMAN

NCBI Description

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

Uniprot Description

TFF1: Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: extracellular space

Molecular Function: growth factor activity; protein binding

Biological Process: carbohydrate metabolic process; cell differentiation; digestion; maintenance of gastrointestinal epithelium; negative regulation of cell proliferation; response to iron ion; response to peptide hormone stimulus