Porcine Angiogenin (ANG) ELISA Kit | ANG elisa kit

Pig Angiogenin (ANG) ELISA Kit

Cat. Num. AAA2700718

• Gene Names: ANG; ALS9; RAA1; HEL168; RNASE4; RNASE5
• Reactivity: Porcine
• Synonyms: Angiogenin (ANG); Pig Angiogenin (ANG) ELISA Kit; RNASE5; Ribonuclease; RNase A Family 5; ANG elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Angiogenin (ANG).; No significant cross-reactivity or interference between Angiogenin (ANG) and analogues was observed.
• Sequence Length: 147

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.625-40ng/mL
• Sensitivity: < 0.257ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiogenin (ANG) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiogenin (ANG) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANG elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiogenin (ANG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiogenin (ANG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiogenin (ANG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiogenin (ANG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557313
• NCBI GeneID: 283
• NCBI Accession #: NP_001136.1
• NCBI GenBank Nucleotide #: NM_001145.4
• UniProt Accession #: P03950; Q05CV1; Q53X86; Q6P5T2; Q8WXE7
• Molecular Weight: 16,550 Da
• NCBI Official Full Name: angiogenin
• NCBI Official Synonym Full Names: angiogenin
• NCBI Official Symbol: ANG
• NCBI Official Synonym Symbols: ALS9; RAA1; HEL168; RNASE4; RNASE5
• NCBI Protein Information: angiogenin
• UniProt Protein Name: Angiogenin
• Protein Family: Angiogenin
• UniProt Gene Name: ANG
• UniProt Synonym Gene Names: RNASE5; RNase 5

NCBI Description

The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2014]

Uniprot Description

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.