NUDT16 sirna

NUDT16 siRNA (Mouse)

Cat. Num. AAA8216808

• Gene Names: Nudt16; AI851783; 2310041H06Rik; 2810047L04Rik; 2900006H04Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NUDT16; NUDT16 siRNA (Mouse); U8 snoRNA-decapping enzyme; IDP phosphatase; IDPase; Inosine diphosphate phosphatase; Nucleoside diphosphate-linked moiety X motif 16; Nudix motif 16; m7GpppN-mRNA hydrolase; NUDT16 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: NUDT16 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 195

• Applicable Applications for NUDT16 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NUDT16 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NUDT16 sirna

siRNA to inhibit NUDT16 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 125656157
• NCBI GeneID: 75686
• NCBI Accession #: NP_083661.2
• NCBI GenBank Nucleotide #: NM_029385.2
• UniProt Accession #: Q6P3D0; Q9D716
• Molecular Weight: 21,825 Da
• NCBI Official Full Name: U8 snoRNA-decapping enzyme
• NCBI Official Synonym Full Names: nudix (nucleoside diphosphate linked moiety X)-type motif 16
• NCBI Official Symbol: Nudt16
• NCBI Official Synonym Symbols: AI851783; 2310041H06Rik; 2810047L04Rik; 2900006H04Rik
• NCBI Protein Information: U8 snoRNA-decapping enzyme
• UniProt Protein Name: U8 snoRNA-decapping enzyme
• Protein Family: Nudix hydrolase
• UniProt Gene Name: Nudt16
• UniProt Synonym Gene Names: IDPase; Nudix motif 16
• UniProt Entry Name: NUD16_MOUSE

Uniprot Description

NUDT16: RNA-decapping enzyme that binds specifically to U8 snoRNA. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and m227G caps from U8 snoRNA. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species (in vitro). Belongs to the Nudix hydrolase family.

Protein type: EC 3.6.1.62; Hydrolase; Nucleolus

Cellular Component: cytoplasm; nucleolus; nucleus

Molecular Function: snoRNA binding; dihydroneopterin monophosphate phosphatase activity; UDP-2,3-diacylglucosamine hydrolase activity; protein homodimerization activity; metalloexopeptidase activity; GTP binding; hydrolase activity; RNA binding; ATP-dependent 5'-3' DNA helicase activity; metal ion binding; nucleotide binding; magnesium ion binding; dATP pyrophosphohydrolase activity; 8-oxo-7,8-dihydroguanine triphosphatase activity; bis(5'-nucleosyl)-tetraphosphatase activity; mRNA binding; m7G(5')pppN diphosphatase activity; pyrophosphatase activity; manganese ion binding; thiamin-pyrophosphatase activity; dihydroneopterin triphosphate pyrophosphohydrolase activity; cobalt ion binding

Biological Process: snoRNA catabolic process; dephosphorylation; positive regulation of cell proliferation; mRNA catabolic process; nucleotide metabolic process; proteolysis; adenosine to inosine editing; chromosome organization and biogenesis; IDP catabolic process