Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using NUDT16 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit NUDT16 Polyclonal Antibody | anti-NUDT16 antibody
NUDT16 Antibody
Reactivity
HumanPredicted: Pig, Bovine, Horse, Sheep
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
NUDT16; Polyclonal Antibody; NUDT16 Antibody; FLJ31265; FLJ34034; FLJ36248; Nucleoside diphosphate-linked moiety X motif 16; NUD16_HUMAN; Nudix (nucleoside diphosphate linked moiety X) type motif 16; Nudix motif 16; NUDT 16; U8 snoRNA-binding protein H29K; U8 snoRNA-decapping enzyme; anti-NUDT16 antibody
Host
Rabbit
Reactivity
Human
Predicted: Pig, Bovine, Horse, Sheep
Predicted: Pig, Bovine, Horse, Sheep
Clonality
Polyclonal
Isotype
IgG
Specificity
NUDT16 Antibody detects endogenous levels of NUDT16.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
159
Applicable Applications for anti-NUDT16 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human NUDT16.
Tissue Specificity
Expressed strongly in lung, kidney, adrenal gland, testis, heart and brain.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using NUDT16 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using NUDT16 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-NUDT16 antibody
Description: RNA-binding and decapping enzyme that catalyzes the cleavage of the cap structure of snoRNAs and mRNAs in a metal-dependent manner. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and/or m227G caps from U8 snoRNA and leaves a 5'monophosphate on the RNA. Catalyzes also the cleavage of the cap structure on mRNAs. Does not hydrolyze cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG). Also hydrolysis m7G- and m227G U3-capped RNAs but with less efficiencies. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species. Binds to the U8 snoRNA; metal is not required for RNA-binding. May play a role in the regulation of snoRNAs and mRNAs degradation. Acts also as a phosphatase; hydrolyzes the non-canonical purine nucleotides inosine diphosphate (IDP) and deoxyinosine diphosphate (dITP) as well as guanosine diphosphate (GDP), deoxyguanosine diphosphate (dGDP), xanthine diphosphate (XDP), inosine triphosphate (ITP) and deoxyinosine triphosphate (ITP) to their respective monophosphate derivatives and does not distinguish between the deoxy- and ribose forms (PubMed:20385596, PubMed:26121039). The order of activity with different substrates is IDP > dIDP >> GDP = dGDP > XDP = ITP = dITP (PubMed:20385596). Binds strongly to GTP, ITP and XTP. Participates in the hydrolysis of dIDP/IDP and probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions (PubMed:20385596).
Subunit Structure: Homodimer.
Similarity: Belongs to the Nudix hydrolase family. NUDT16 subfamily.
Subunit Structure: Homodimer.
Similarity: Belongs to the Nudix hydrolase family. NUDT16 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 21 kDa
Predicted: 22 kDa
Predicted: 22 kDa
NCBI Official Full Name
U8 snoRNA-decapping enzyme isoform 3
NCBI Official Synonym Full Names
nudix hydrolase 16
NCBI Official Symbol
NUDT16
NCBI Protein Information
U8 snoRNA-decapping enzyme
UniProt Protein Name
U8 snoRNA-decapping enzyme
Protein Family
UniProt Gene Name
NUDT16
UniProt Synonym Gene Names
IDPase; Nudix motif 16
UniProt Entry Name
NUD16_HUMAN
Uniprot Description
NUDT16: RNA-decapping enzyme that binds specifically to U8 snoRNA. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and m227G caps from U8 snoRNA. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species (in vitro). Belongs to the Nudix hydrolase family.
Protein type: Hydrolase; Nucleolus; EC 3.6.1.62
Chromosomal Location of Human Ortholog: 3q22.1
Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus
Molecular Function: mRNA binding; snoRNA binding; m7G(5')pppN diphosphatase activity; protein homodimerization activity; GTP binding; metalloexopeptidase activity; manganese ion binding; magnesium ion binding; cobalt ion binding
Biological Process: snoRNA catabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; positive regulation of cell proliferation; mRNA catabolic process; proteolysis; adenosine to inosine editing; IDP catabolic process
Research Articles on NUDT16
Similar Products
Product Notes
The NUDT16 nudt16 (Catalog #AAA9611948) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NUDT16 Antibody reacts with Human Predicted: Pig, Bovine, Horse, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's NUDT16 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the NUDT16 nudt16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUDT16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
