Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NUDT16 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)
Rabbit anti-Human, Mouse NUDT16 Polyclonal Antibody | anti-NUDT16 antibody
NUDT16 Rabbit pAb
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NUDT16 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NUDT16 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)
NCBI and Uniprot Product Information
Uniprot Description
NUDT16: RNA-decapping enzyme that binds specifically to U8 snoRNA. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and m227G caps from U8 snoRNA. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species (in vitro). Belongs to the Nudix hydrolase family.
Protein type: Hydrolase; Nucleolus; EC 3.6.1.62
Chromosomal Location of Human Ortholog: 3q22.1
Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus
Molecular Function: mRNA binding; snoRNA binding; m7G(5')pppN diphosphatase activity; protein homodimerization activity; GTP binding; metalloexopeptidase activity; manganese ion binding; magnesium ion binding; cobalt ion binding
Biological Process: snoRNA catabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; positive regulation of cell proliferation; mRNA catabolic process; proteolysis; adenosine to inosine editing; IDP catabolic process
Research Articles on NUDT16
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Product Notes
The NUDT16 nudt16 (Catalog #AAA9143047) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NUDT16 Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NUDT16 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the NUDT16 nudt16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GFVDTQDRSL EDGLNRELRE ELGEAAAAFR VERTDYRSSH VGSGPRVVAH FYAKRLTLEE LLAVEAGATR A. It is sometimes possible for the material contained within the vial of "NUDT16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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