NEIL3 sirna

NEIL3 siRNA (Mouse)

Cat. Num. AAA8227515

• Gene Names: Neil3; C85903; AI449477; BC034753
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NEIL3; NEIL3 siRNA (Mouse); Endonuclease 8-like 3; DNA glycosylase FPG2; DNA glycosylase/AP lyase Neil3; Endonuclease VIII-like 3; Nei-like protein 3; NEIL3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: NEIL3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 211

• Applicable Applications for NEIL3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NEIL3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NEIL3 sirna

siRNA to inhibit NEIL3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 22122759
• NCBI GeneID: 234258
• NCBI Accession #: NP_666320.1
• NCBI GenBank Nucleotide #: NM_146208.2
• UniProt Accession #: Q8K203; Q4ADY6; Q8CD85; Q8R3P4
• Molecular Weight: 22,840 Da
• NCBI Official Full Name: endonuclease 8-like 3
• NCBI Official Synonym Full Names: nei like 3 (E. coli)
• NCBI Official Symbol: Neil3
• NCBI Official Synonym Symbols: C85903; AI449477; BC034753
• NCBI Protein Information: endonuclease 8-like 3
• UniProt Protein Name: Endonuclease 8-like 3
• Protein Family: Endonuclease
• UniProt Gene Name: Neil3
• UniProt Entry Name: NEIL3_MOUSE

Uniprot Description

NEIL3: Reports about DNA glycosylase activity are contradictory. A number of references report finding no DNA glycosylase activity and the protein lacks a proline residue at the N-terminus which functions as an active site residue in other members of the FPG family. However, the mouse ortholog has been shown to have both DNA glycosylase and AP lyase activities and PubMed:19170771 demonstrates AP lyase activity. Prefers single- stranded DNA or partially single-stranded DNA structures such as bubble and fork structures to double-stranded DNA in vitro. Displays a broad recognition spectrum, preferring FapyA and FapyG followed by 5-OHU, 5-PHC and 5-OHMH and then Tg and 8-oxoA. No activity on 8-oxoG detected. Belongs to the FPG family.

Protein type: EC 4.2.99.18; DNA-binding

Cellular Component: nucleus

Molecular Function: single-strand selective uracil DNA N-glycosylase activity; hydrolase activity, hydrolyzing N-glycosyl compounds; DNA-(apurinic or apyrimidinic site) lyase activity; uracil DNA N-glycosylase activity; hydrolase activity; zinc ion binding; metal ion binding; DNA N-glycosylase activity; lyase activity; hydrolase activity, acting on glycosyl bonds; purine-specific mismatch base pair DNA N-glycosylase activity; bubble DNA binding; DNA-3-methyladenine glycosylase III activity; DNA binding; nucleic acid binding; double-stranded DNA binding; damaged DNA binding; catalytic activity; single-stranded DNA binding

Biological Process: nucleotide-excision repair; metabolic process; base-excision repair; DNA repair; response to DNA damage stimulus; DNA catabolic process, endonucleolytic