NCEH1 sirna

NCEH1 siRNA (Mouse)

Cat. Num. AAA8209479

• Gene Names: Nceh1; Nceh; CPO-BP; Aadacl1; B230106I24Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NCEH1; NCEH1 siRNA (Mouse); AADACL1; KIAA1363; Neutral cholesterol ester hydrolase 1; NCEH; Arylacetamide deacetylase-like 1; Chlorpyrifos oxon-binding protein; CPO-BP; NCEH1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: NCEH1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 408

• Applicable Applications for NCEH1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NCEH1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NCEH1 sirna

siRNA to inhibit NCEH1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 30520239
• NCBI GeneID: 320024
• NCBI Accession #: NP_848887.1
• NCBI GenBank Nucleotide #: NM_178772.3
• UniProt Accession #: Q8BLF1; Q69ZL0; Q8BZK3
• Molecular Weight: 45,740 Da
• NCBI Official Full Name: neutral cholesterol ester hydrolase 1
• NCBI Official Synonym Full Names: neutral cholesterol ester hydrolase 1
• NCBI Official Symbol: Nceh1
• NCBI Official Synonym Symbols: Nceh; CPO-BP; Aadacl1; B230106I24Rik
• NCBI Protein Information: neutral cholesterol ester hydrolase 1
• UniProt Protein Name: Neutral cholesterol ester hydrolase 1
• Protein Family: Neutral cholesterol ester hydrolase
• UniProt Gene Name: Nceh1
• UniProt Synonym Gene Names: Aadacl1; Kiaa1363; NCEH; CPO-BP
• UniProt Entry Name: NCEH1_MOUSE

Uniprot Description

AADACL1: a single-pass type II membrane microsomal protein. Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (acetyl-glyceryl-ether-phosphorylcholine). Elevated in invasive cancer cells from several different tumor types. May be involved in the elevated neutral ether lipid content and elevated LPA signaling in cancer cells. May be responsible for cholesterol ester hydrolysis in macrophages, thereby contributing to the development of atherosclerosis. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May contribute to cancer pathogenesis by promoting tumor cell migration. A key enzymatic node regulating an ether lipid signaling network in cancer; regulates MAGE and lysophospholipid levels in invasive cancer cells. Three alternatively spliced human isoforms have been described.

Protein type: Membrane protein, integral; EC 3.1.1.-; Hydrolase

Cellular Component: intracellular membrane-bound organelle; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: sulfolactone hydrolase activity; retinyl-palmitate esterase activity; serine hydrolase activity; 3,4-dihydrocoumarin hydrolase activity; hydrolase activity; lipase activity; butyrolactone hydrolase activity; all-trans-retinyl-palmitate hydrolase activity; phosphate binding; phospholipase activity; 1-oxa-2-oxocycloheptane lactonase activity; prenylcysteine methylesterase activity

Biological Process: metabolic process; xenobiotic metabolic process; lipid metabolic process; protein amino acid dephosphorylation; lipid catabolic process