MNAT1 sirna

MNAT1 siRNA (Mouse)

Cat. Num. AAA8220897

• Gene Names: Mnat1; P36; MAT1; E130115E11Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MNAT1; MNAT1 siRNA (Mouse); MAT1; CDK-activating kinase assembly factor MAT1; CDK7/cyclin-H assembly factor; Menage a trois; RING finger protein MAT1; p35; p36; MNAT1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MNAT1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 309

• Applicable Applications for MNAT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MNAT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MNAT1 sirna

siRNA to inhibit MNAT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 89363038
• NCBI GeneID: 17420
• NCBI Accession #: NP_032638.2
• NCBI GenBank Nucleotide #: NM_008612.2
• UniProt Accession #: P51949; Q14BS9; Q3TZP0; Q9D8A0; Q9D8D2
• Molecular Weight: 35,848 Da
• NCBI Official Full Name: CDK-activating kinase assembly factor MAT1
• NCBI Official Synonym Full Names: menage a trois 1
• NCBI Official Symbol: Mnat1
• NCBI Official Synonym Symbols: P36; MAT1; E130115E11Rik
• NCBI Protein Information: CDK-activating kinase assembly factor MAT1
• UniProt Protein Name: CDK-activating kinase assembly factor MAT1
• Protein Family: CDK-activating kinase assembly factor
• UniProt Gene Name: Mnat1
• UniProt Synonym Gene Names: Mat1
• UniProt Entry Name: MAT1_MOUSE

Uniprot Description

MNAT1: Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.

Protein type: Ubiquitin conjugating system; Ubiquitin ligase; Cell cycle regulation; Nuclear receptor co-regulator

Cellular Component: nucleoplasm; holo TFIIH complex; cytoplasm; nucleus

Molecular Function: RNA polymerase subunit kinase activity; DNA-dependent ATPase activity; protein binding; zinc ion binding; metal ion binding; protein N-terminus binding

Biological Process: adult heart development; transcription from RNA polymerase II promoter; transcription, DNA-dependent; ventricular system development; positive regulation of smooth muscle cell proliferation; DNA repair; cell cycle; protein amino acid phosphorylation; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; regulation of cyclin-dependent protein kinase activity; response to calcium ion; G1/S transition of mitotic cell cycle; negative regulation of apoptosis