Rabbit anti-Human WW Domain Binding Protein 2 Polyclonal Antibody | anti-WBP2NL antibody

WW Domain Binding Protein 2, NT (WBP2 N-terminal Like, WBP2NL, FLJ26145, MGC26816, OTTHUMP00000028745, Postacrosomal Sheath WW Domain Binding Protein, PAWP) (AP)

Cat. Num. AAA6506246

• Gene Names: WBP2; WBP-2; GRAMD6; DFNB107
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: WW Domain Binding Protein 2; Polyclonal Antibody; NT (WBP2 N-terminal Like; WBP2NL; FLJ26145; MGC26816; OTTHUMP00000028745; Postacrosomal Sheath WW Domain Binding Protein; PAWP) (AP); anti-WBP2NL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human WBP2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-WBP2NL antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human WBP2 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-WBP2 Antibody (N-term) in A2058 cell line lysates (35ug/lane). WBP2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(WBP2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WBP2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-WBP2NL antibody

Antibodies; Cell Markers

References

1. Dhananjayan, S.C., Mol. Endocrinol. 20(10), 2343-2354 (2006). 2. Nitsch, R., Biochem. J. 377(PT 3), 553-560 (2004). 3. Jolliffe, C.N., Biochem. J. 351PT 3, 557-565 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 24430132
• NCBI GeneID: 23558
• NCBI Accession #: NP_036610
• NCBI GenBank Nucleotide #: NM_012478.3
• UniProt Accession #: Q969T9; O95638; B4DFG2
• Molecular Weight: 30.5 kDa (284aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: WW domain-binding protein 2 isoform 1
• NCBI Official Synonym Full Names: WW domain binding protein 2
• NCBI Official Symbol: WBP2
• NCBI Official Synonym Symbols: WBP-2; GRAMD6; DFNB107
• NCBI Protein Information: WW domain-binding protein 2
• UniProt Protein Name: WW domain-binding protein 2
• Protein Family: Postacrosomal sheath WW domain-binding protein
• UniProt Gene Name: WBP2
• UniProt Synonym Gene Names: WBP-2
• UniProt Entry Name: WBP2_HUMAN

NCBI Description

The globular WW domain is composed of 38 to 40 semiconserved amino acids shared by proteins of diverse functions including structural, regulatory, and signaling proteins. The domain is involved in mediating protein-protein interactions through the binding of polyproline ligands. This gene encodes a WW domain binding protein that is a transcriptional coactivator of estrogen receptor alpha and progesterone receptor. Defects in this gene have been associated with hearing impairment. [provided by RefSeq, Jan 2017]

Uniprot Description

WBP2: Binds to the WW domain of YAP1, WWP1 and WWP2. Interacts with NEDD4.

Protein type: Nuclear receptor co-regulator; Cell development/differentiation

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: nuclear chromatin

Molecular Function: protein binding; chromatin DNA binding

Biological Process: establishment of protein localization; positive regulation of gene expression, epigenetic; positive regulation of transcription from RNA polymerase II promoter