MBD4 sirna

MBD4 siRNA (Mouse)

Cat. Num. AAA828877

• Gene Names: Mbd4; Med1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MBD4; MBD4 siRNA (Mouse); Methyl-CpG-binding domain protein 4; Methyl-CpG-binding protein MBD4; Mismatch-specific DNA N-glycosylase; MBD4 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MBD4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 554

• Applicable Applications for MBD4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MBD4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MBD4 sirna

siRNA to inhibit MBD4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 187960081
• NCBI GeneID: 17193
• NCBI Accession #: NP_034904.2
• NCBI GenBank Nucleotide #: NM_010774.2
• UniProt Accession #: Q9Z2D7; Q792D2; Q8R3R3
• Molecular Weight: 62,578 Da
• NCBI Official Full Name: methyl-CpG-binding domain protein 4
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 4
• NCBI Official Symbol: Mbd4
• NCBI Official Synonym Symbols: Med1
• NCBI Protein Information: methyl-CpG-binding domain protein 4
• UniProt Protein Name: Methyl-CpG-binding domain protein 4
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: Mbd4
• UniProt Entry Name: MBD4_MOUSE

Uniprot Description

MBD4: Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.2.2.-; DNA repair, damage; Apoptosis; Deoxyribonuclease

Cellular Component: nucleoplasm; cytoplasm; intracellular; chromatin; nucleus

Molecular Function: single-strand selective uracil DNA N-glycosylase activity; DNA-3-methyladenine glycosylase III activity; uracil DNA N-glycosylase activity; DNA binding; hydrolase activity; DNA N-glycosylase activity; purine-specific mismatch base pair DNA N-glycosylase activity; catalytic activity; pyrimidine-specific mismatch base pair DNA N-glycosylase activity

Biological Process: response to radiation; DNA damage response, signal transduction resulting in induction of apoptosis; base-excision repair; mitotic cell cycle G2/M transition DNA damage checkpoint; DNA methylation; DNA repair; response to DNA damage stimulus