MAPK1 sirna

MAPK1 siRNA (Mouse)

Cat. Num. AAA8211728

• Gene Names: Mapk1; ERK; Erk2; MAPK2; PRKM2; Prkm1; C78273; p41mapk; p42mapk; AA407128; AU018647; 9030612K14Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAPK1; MAPK1 siRNA (Mouse); ERK2; MAPK; PRKM1; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; MAPK1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MAPK1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 358

• Applicable Applications for MAPK1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MAPK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAPK1 sirna

siRNA to inhibit MAPK1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 84579909
• NCBI GeneID: 26413
• NCBI Accession #: NP_001033752.1
• NCBI GenBank Nucleotide #: NM_001038663.1
• UniProt Accession #: P63085; P27703; Q3V1U6
• Molecular Weight: 41,276 Da
• NCBI Official Full Name: mitogen-activated protein kinase 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 1
• NCBI Official Symbol: Mapk1
• NCBI Official Synonym Symbols: ERK; Erk2; MAPK2; PRKM2; Prkm1; C78273; p41mapk; p42mapk; AA407128; AU018647; 9030612K14Rik
• NCBI Protein Information: mitogen-activated protein kinase 1
• UniProt Protein Name: Mitogen-activated protein kinase 1
• UniProt Gene Name: Mapk1
• UniProt Synonym Gene Names: Erk2; Mapk; Prkm1; MAP kinase 1; MAPK 1; ERK-2; p42-MAPK; MAP kinase 2; MAPK 2
• UniProt Entry Name: MK01_MOUSE

Uniprot Description

ERK2: a serine/threonine kinase of the GMGC group that plays a critical role in the regulation of cell growth and differentiation. ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Depending on the cellular context, MAPK cascades mediate diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. MAPK cascades also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine residues at the sequence T*EY* by upstream MAP kinase kinases, MEK1 and -2. Phosphorylation of both the threonine and tyrosine are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; EC 2.7.11.24; CMGC group; MAPK family; MAPK/ERK subfamily; ERK subfamily

Cellular Component: dendrite cytoplasm; Golgi apparatus; focal adhesion; protein complex; mitochondrion; early endosome; perikaryon; pseudopodium; caveola; cytosol; nucleoplasm; microtubule cytoskeleton; cytoskeleton; axon; cytoplasm; late endosome; nucleus

Molecular Function: nucleotide binding; mitogen-activated protein kinase kinase kinase binding; transcription factor binding; protein kinase binding; protein kinase activity; phosphatase binding; RNA polymerase subunit kinase activity; MAP kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; phosphotyrosine binding; transferase activity, transferring phosphorus-containing groups; kinase activity; ATP binding

Biological Process: positive regulation of translation; apoptosis; response to toxin; response to lipopolysaccharide; signal transduction; sensory perception of pain; T cell receptor signaling pathway; protein amino acid phosphorylation; B cell receptor signaling pathway; response to exogenous dsRNA; regulation of transcription factor activity; lipopolysaccharide-mediated signaling pathway; transcription, DNA-dependent; peptidyl-threonine phosphorylation; MAPKKK cascade; mammary gland epithelial cell proliferation; cell cycle; regulation of stress-activated MAPK cascade; organ morphogenesis; nuclear translocation of MAPK; peptidyl-serine phosphorylation; negative regulation of cell differentiation; cytosine metabolic process; response to estrogen stimulus; response to DNA damage stimulus; phosphorylation; regulation of cytoskeleton organization and biogenesis