MAP3K3 sirna

MAP3K3 siRNA (Mouse)

Cat. Num. AAA8211697

• Gene Names: Map3k3; Mekk3; MAPKKK3; AW548911; mKIAA4031
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAP3K3; MAP3K3 siRNA (Mouse); MEKK3; Mitogen-activated protein kinase kinase kinase 3; MAPK/ERK kinase kinase 3; MEK kinase 3; MEKK 3; MAP3K3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MAP3K3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 626

• Applicable Applications for MAP3K3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MAP3K3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAP3K3 sirna

siRNA to inhibit MAP3K3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 33468949
• NCBI GeneID: 26406
• NCBI Accession #: NP_036077.1
• NCBI GenBank Nucleotide #: NM_011947.3
• UniProt Accession #: Q61084
• Molecular Weight: 70,776 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 3
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 3
• NCBI Official Symbol: Map3k3
• NCBI Official Synonym Symbols: Mekk3; MAPKKK3; AW548911; mKIAA4031
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 3
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 3
• UniProt Gene Name: Map3k3
• UniProt Synonym Gene Names: Mekk3; MEK kinase 3; MEKK 3
• UniProt Entry Name: M3K3_MOUSE

Uniprot Description

MEKK3: a protein kinase of the STE11 family. Phosphorylated and activated by MLK3 and TAK1. Phosphorylates and activates MEK5.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.25; Protein kinase, STE; STE group; STE11 family

Cellular Component: intracellular

Molecular Function: MAP kinase kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; metal ion binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: blood vessel development; activation of MAPK activity; protein amino acid autophosphorylation; phosphorylation; protein amino acid phosphorylation