Rabbit anti-Mouse, Rat MEKK3 Polyclonal Antibody | anti-MAP3K3 antibody

Anti-MEKK3 antibody

Cat. Num. AAA176013

• Gene Names: Map3k3; Mekk3; MAPKKK3; AW548911; mKIAA4031
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: MEKK3; Polyclonal Antibody; Anti-MEKK3 antibody; Mitogen-activated protein kinase kinase kinase 3; mitogen-activated protein kinase kinase kinase 3; M3K3_HUMAN antibody; MAP/ERK kinase kinase 3 antibody; MAP3K3 antibody; MAPK/ERK kinase kinase 3 antibody; MAPKKK3 antibody; MEK kinase 3 antibody; MEKK 3 antibody; MEKK3 antibody; mitogen activated protein kinase kinase kinase 3 antibody; Mitogen-activated protein kinase kinase kinase 3 antibody; anti-MAP3K3 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 626

• Applicable Applications for anti-MAP3K3 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse MEKK3 (11-25aa MKDLVALQMSRRTRL), different from the related rat sequence by two amino acids.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-MEKK3 antibody, MBS176013, IHC(P)IHC(P): Rat Brain Tissue )

Immunohistochemistry (IHC)

(Anti-MEKK3 antibody, MBS176013, IHC(P)IHC(P): Rat Brain Tissue )

Western Blot (WB)

(Anti-MEKK3 antibody, MBS176013, Western blottingAll lanes: Anti MAP3K3 (MBS176013) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Mouse Brain Tissue Lysate at 50ugPredicted bind size: 71KDObserved bind size: 71KD )

Related Product Information for anti-MAP3K3 antibody

Description: Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase kinase kinase 3(MAP3K3) detection. Tested with WB, IHC-P in Mouse, Rat.
Background: MAP3K3(Mitogen-activated protein kinase kinase kinase 3), also known as MAP/ERK KINASE KINASE 3, MEKK3 or MAPKKK3, is an enzyme that in humans is encoded by the MAP3K3 gene. MAP3K3 is contiguously distal to LYK5 on chromosome 17(Puffenberger et al., 2007). By yeast 2-hybrid analysis of a mouse T-cell cDNA library, Uhlik et al.(2003) showed that a C-terminal fragment of mouse Osm(CCM2) interacted with Mekk3, which activates p38 in response to sorbitol-induced hyperosmotic conditions. Mekk3 and Osm colocalized in the cytoplasmic compartment of cotransfected cells, and the Mekk3-Osm complex was recruited to Rac1 and cytoskeletal actin-containing membrane ruffles in response to sorbitol treatment. Protein interaction assays showed that Osm interacted directly with the Mekk3 substrate Mkk3(MAP2K3), with actin, and with both GDP- and GTP-loaded Rac1. Uhlik et al.(2003) concluded that the RAC1-OSM-MEKK3-MKK3 complex is required for regulation of p38 activity in response to osmotic shock.

NCBI and Uniprot Product Information

• NCBI GI #: 2499641
• NCBI GeneID: 26406
• UniProt Accession #: Q61084
• Molecular Weight: 70,776 Da
• NCBI Official Full Name: Mitogen-activated protein kinase kinase kinase 3
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 3
• NCBI Official Symbol: Map3k3
• NCBI Official Synonym Symbols: Mekk3; MAPKKK3; AW548911; mKIAA4031
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 3; MEKK 3; MEK kinase 3; MAPK/ERK kinase kinase 3; mitogen activated protein kinase kinase kinase 3
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 3
• UniProt Gene Name: Map3k3
• UniProt Synonym Gene Names: Mekk3; MEK kinase 3; MEKK 3
• UniProt Entry Name: M3K3_MOUSE

Uniprot Description

MEKK3: a protein kinase of the STE11 family. Phosphorylated and activated by MLK3 and TAK1. Phosphorylates and activates MEK5.

Protein type: Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.25; STE group; STE11 family

Cellular Component: intracellular

Molecular Function: transferase activity; MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; metal ion binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: blood vessel development; activation of MAPK activity; protein amino acid autophosphorylation; protein amino acid phosphorylation; phosphorylation