MAOB sirna

MAOB siRNA (Mouse)

Cat. Num. AAA8210062

• Gene Names: Maob; MAO-B; 6330414K01Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAOB; MAOB siRNA (Mouse); Amine oxidase [flavin-containing] B; Monoamine oxidase type B; MAO-B; MAOB sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MAOB siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 520

• Applicable Applications for MAOB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MAOB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAOB sirna

siRNA to inhibit MAOB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 257196228
• NCBI GeneID: 109731
• NCBI Accession #: NP_766366.2
• NCBI GenBank Nucleotide #: NM_172778.2
• UniProt Accession #: Q8BW75; Q14CG9; Q8C0B2
• Molecular Weight: 58,558 Da
• NCBI Official Full Name: amine oxidase
• NCBI Official Synonym Full Names: monoamine oxidase B
• NCBI Official Symbol: Maob
• NCBI Official Synonym Symbols: MAO-B; 6330414K01Rik
• NCBI Protein Information: amine oxidase [flavin-containing] B
• UniProt Protein Name: Amine oxidase [flavin-containing] B
• Protein Family: Amine oxidase
• UniProt Gene Name: Maob
• UniProt Synonym Gene Names: MAO-B
• UniProt Entry Name: AOFB_MOUSE

Uniprot Description

MAOB: Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine. Belongs to the flavin monoamine oxidase family.

Protein type: Amino Acid Metabolism - phenylalanine; Mitochondrial; Amino Acid Metabolism - glycine, serine and threonine; Amino Acid Metabolism - histidine; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - tryptophan; Oxidoreductase; Membrane protein, integral; EC 1.4.3.4; Xenobiotic Metabolism - drug metabolism - cytochrome P450

Cellular Component: mitochondrial outer membrane; mitochondrion; membrane; mitochondrial inner membrane; integral to membrane

Molecular Function: amine oxidase activity; protein homodimerization activity; FAD binding; oxidoreductase activity

Biological Process: negative regulation of serotonin secretion; positive regulation of dopamine metabolic process; neurotransmitter catabolic process