Rabbit anti-Human, Mouse COQ5 Polyclonal Antibody | anti-COQ5 antibody

COQ5, ID (COQ5, 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial, Ubiquinone biosynthesis methyltransferase COQ5) (Azide free) (HRP)

Cat. Num. AAA6287937

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: COQ5; Polyclonal Antibody; ID (COQ5; 2-methoxy-6-polyprenyl-1; 4-benzoquinol methylase; mitochondrial; Ubiquinone biosynthesis methyltransferase COQ5) (Azide free) (HRP); Ubiquinone biosynthesis methyltransferase COQ5; anti-COQ5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-COQ5 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: COQ5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220~249 amino acids from the Center region of human COQ5.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of COQ5 Antibody (Center) in mouse heart tissue lysates (35ug/lane). COQ5 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(COQ5 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COQ5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-COQ5 antibody

Antibodies; Enzymes; Methyltransferase

References

Suzuki, Y., et.al., Genome Res. 14 (9), 1711-1718 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 189032253
• NCBI GeneID: 84274
• UniProt Accession #: Q5HYK3; Q32Q28; Q53HH0; Q96LV1; Q9BSP8; B4DEJ4
• Molecular Weight: 37,140 Da
• NCBI Official Full Name: COQ5
• NCBI Official Synonym Full Names: coenzyme Q5 homolog, methyltransferase (S. cerevisiae)
• NCBI Official Symbol: COQ5
• NCBI Protein Information: 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial; ubiquinone biosynthesis methyltransferase COQ5, mitochondrial
• UniProt Protein Name: 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial
• Protein Family: 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase
• UniProt Gene Name: COQ5
• UniProt Entry Name: COQ5_HUMAN

Uniprot Description

Function: Methyltransferase required for the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)

By similarity.

Catalytic activity: S-adenosyl-L-methionine + 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol = S-adenosyl-L-homocysteine + 6-methoxy-3-methyl-2-all-trans-polyprenyl-1,4-benzoquinol.

Pathway: Cofactor biosynthesis; ubiquinone biosynthesis.

Subcellular location: Mitochondrion

By similarity.

Sequence similarities: Belongs to the class I-like SAM-binding methyltransferase superfamily. UbiE family.