MAGED1 sirna

MAGED1 siRNA (Mouse)

Cat. Num. AAA8235730

• Gene Names: Maged1; NRAGE; Dlixin; Dlxin1; Dlxin-1; MAGE-D1; mFLJ00163; DXBwg1492e; 2810433C11Rik; 5430405L04Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAGED1; MAGED1 siRNA (Mouse); NRAGE; Melanoma-associated antigen D1; Dlxin-1; MAGE-D1 antigen; Neurotrophin receptor-interacting MAGE homolog; MAGED1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MAGED1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 775

• Applicable Applications for MAGED1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MAGED1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAGED1 sirna

siRNA to inhibit MAGED1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 9789935
• NCBI GeneID: 94275
• NCBI Accession #: NP_062765.1
• NCBI GenBank Nucleotide #: NM_019791.2
• UniProt Accession #: Q9QYH6; Q543L6; Q99PB5; Q9CYX1
• Molecular Weight: 85,670 Da
• NCBI Official Full Name: melanoma-associated antigen D1
• NCBI Official Synonym Full Names: melanoma antigen, family D, 1
• NCBI Official Symbol: Maged1
• NCBI Official Synonym Symbols: NRAGE; Dlixin; Dlxin1; Dlxin-1; MAGE-D1; mFLJ00163; DXBwg1492e; 2810433C11Rik; 5430405L04Rik
• NCBI Protein Information: melanoma-associated antigen D1
• UniProt Protein Name: Melanoma-associated antigen D1
• Protein Family: Melanoma-associated antigen
• UniProt Gene Name: Maged1
• UniProt Synonym Gene Names: Nrage
• UniProt Entry Name: MAGD1_MOUSE

Uniprot Description

NRAGE: a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although NRAGE shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. Binds p75 neurotrophin receptor (p75NTR) and antagonizes its association with TrkA, inhibits cell cycle progression, and facilitates p75NTR-mediated apoptosis. May act as a regulator of the function of DLX family members.

Protein type: Adaptor/scaffold

Cellular Component: protein complex; membrane; cytoplasm; plasma membrane; chromatin; nucleus

Molecular Function: protein binding; transcription coactivator activity

Biological Process: regulation of apoptosis; regulation of transcription from RNA polymerase II promoter; positive regulation of MAP kinase activity; positive regulation of transcription, DNA-dependent; rhythmic process; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent; regulation of circadian rhythm; negative regulation of epithelial cell proliferation