LOX sirna

LOX siRNA (Mouse)

Cat. Num. AAA8234575

• Gene Names: Lox; rrg; TSC-160; AI893619
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LOX; LOX siRNA (Mouse); RRG; Protein-lysine 6-oxidase; Lysyl oxidase; Ras excision protein; LOX sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: LOX siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 411

• Applicable Applications for LOX sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse LOX gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LOX sirna

siRNA to inhibit LOX expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 555943929
• NCBI GeneID: 16948
• NCBI Accession #: NP_001273110.1
• NCBI GenBank Nucleotide #: NM_001286181.1
• UniProt Accession #: P28301
• Molecular Weight: 46,701 Da
• NCBI Official Full Name: protein-lysine 6-oxidase preproprotein
• NCBI Official Synonym Full Names: lysyl oxidase
• NCBI Official Symbol: Lox
• NCBI Official Synonym Symbols: rrg; TSC-160; AI893619
• NCBI Protein Information: protein-lysine 6-oxidase
• UniProt Protein Name: Protein-lysine 6-oxidase
• Protein Family: Linoleate 9/13-lipoxygenase
• UniProt Gene Name: Lox
• UniProt Synonym Gene Names: Rrg
• UniProt Entry Name: LYOX_MOUSE

NCBI Description

This gene encodes a precursor protein that belongs to the lysyl oxidase family of proteins. The secreted proprotein is proteolytically processed to an active mature peptide and a propeptide. This propeptide is thought to function in tumor suppression by inhibiting the Ras signaling pathway. The active enzyme plays a role in cross-linking of collagen and elastin and is essential for development of cardiovascular and respiratory systems, and development of skin and connective tissue. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]

Uniprot Description

LOX: Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. Belongs to the lysyl oxidase family.

Protein type: Secreted, signal peptide; EC 1.4.3.13; Secreted; Oxidoreductase

Cellular Component: proteinaceous extracellular matrix; collagen; extracellular region; nucleus

Molecular Function: protein binding; copper ion binding; protein-lysine 6-oxidase activity; metal ion binding; oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor; oxidoreductase activity

Biological Process: elastic fiber assembly; blood vessel development; collagen fibril organization; wound healing; response to steroid hormone stimulus; response to hormone stimulus; lung development