KIT sirna

KIT siRNA (Mouse)

Cat. Num. AAA8224470

• Gene Names: Kit; W; Bs; Fdc; Ssm; SCO1; SCO5; SOW3; CD117; c-KIT; Tr-kit; Gsfsco1; Gsfsco5; Gsfsow3
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KIT; KIT siRNA (Mouse); SL; Mast/stem cell growth factor receptor Kit; SCFR; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; CD antigen CD117; KIT sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: KIT siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 979

• Applicable Applications for KIT sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse KIT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KIT sirna

siRNA to inhibit KIT expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 170650721
• NCBI GeneID: 16590
• NCBI Accession #: NP_001116205.1
• NCBI GenBank Nucleotide #: NM_001122733.1
• UniProt Accession #: P05532; Q61415; Q61416; Q61417; Q6LEE9; Q6QJB7; Q6QJB8; Q7TS86; Q8C8K9
• Molecular Weight: 22,814 Da
• NCBI Official Full Name: mast/stem cell growth factor receptor Kit isoform 1
• NCBI Official Synonym Full Names: kit oncogene
• NCBI Official Symbol: Kit
• NCBI Official Synonym Symbols: W; Bs; Fdc; Ssm; SCO1; SCO5; SOW3; CD117; c-KIT; Tr-kit; Gsfsco1; Gsfsco5; Gsfsow3
• NCBI Protein Information: mast/stem cell growth factor receptor Kit
• UniProt Protein Name: Mast/stem cell growth factor receptor Kit
• Protein Family: Kit ligand
• UniProt Gene Name: Kit
• UniProt Synonym Gene Names: Sl; SCFR
• UniProt Entry Name: KIT_MOUSE

NCBI Description

The c-Kit proto-oncogene is the cellular homolog of the transforming gene of a feline retrovirus (v-Kit). The c-kit protein includes characteristics of a protein kinase transmembrane receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Kit: a receptor tyrosine kinase and a member of the subfamily that includes PDGF, CSF-1 and FLT-3/flk-2 receptors. Receptor for stem cell factor. Plays a critical role in hematopoietic stem cell, mast cell, melanocyte and germ cell development. Ligand binding induces autophosphorylation, dimerization and activation, leading to the recruitment and phosphorylation of downstream SH2-containing signaling components including PLC-gamma, PI3 kinase p85, SHP2 and CrkL, linking c-Kit to various cell signaling pathways. Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders, while mutations that constitutively activate c-Kit can lead to hyperplasia and tumorigenesis. Activating mutations cause >90% of gastrointestinal stromal tumors (GIST); successfully treated with inhibitors Gleevec (imatinib, Glivec) and Sutent (Sutinib, SU11248). Activating mutations also induce mastocytosis. Autocrine/paracrine stimulation may drive some lung and other tumors. Loss of expression associated with melanoma progression. Familial loss of function mutations cause piebaldism, with defects in hair and skin pigmentation due to lack of melanocytes.

Protein type: Kinase, protein; Membrane protein, integral; EC 2.7.10.1; Protein kinase, TK; Oncoprotein; Protein kinase, tyrosine (receptor); TK group; PDGFR family

Cellular Component: extracellular space; internal side of plasma membrane; mast cell granule; cell surface; membrane; cytoplasm; acrosome; plasma membrane; integral to membrane; intercellular junction; intracellular; external side of plasma membrane

Molecular Function: protein homodimerization activity; protease binding; metal ion binding; nucleotide binding; stem cell factor receptor activity; transmembrane receptor protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; protein kinase activity; transferase activity; protein binding; cytokine binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; kinase activity; ATP binding

Biological Process: somatic stem cell maintenance; activation of MAPK activity; germ cell programmed cell death; positive regulation of JAK-STAT cascade; lymphoid progenitor cell differentiation; positive regulation of long-term neuronal synaptic plasticity; protein amino acid phosphorylation; germ cell development; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of cell shape; positive regulation of MAP kinase activity; epithelial cell proliferation; germ cell migration; erythrocyte differentiation; somatic stem cell division; T cell differentiation; mast cell chemotaxis; embryonic hemopoiesis; stem cell differentiation; detection of mechanical stimulus involved in sensory perception of sound; positive regulation of tyrosine phosphorylation of Stat1 protein; regulation of pigmentation during development; positive regulation of transcription factor activity; glycosphingolipid metabolic process; immature B cell differentiation; mast cell cytokine production; transmembrane receptor protein tyrosine kinase signaling pathway; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; pigmentation during development; chemotaxis; response to radiation; ovarian follicle development; myeloid progenitor cell differentiation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; melanocyte differentiation; negative regulation of programmed cell death; visual learning; hemopoiesis; inflammatory response; cell differentiation; positive regulation of Notch signaling pathway; lamellipodium biogenesis; dendritic cell cytokine production; cytokine and chemokine mediated signaling pathway; mast cell degranulation; positive regulation of pseudopodium formation; gut development; pigmentation; positive regulation of tyrosine phosphorylation of Stat5 protein; actin cytoskeleton reorganization; myeloid leukocyte differentiation; spermatogenesis; phosphorylation; spermatid development; positive regulation of cell migration