KDM7A sirna

KDM7A siRNA (Mouse)

Cat. Num. AAA8241104

• Gene Names: Kdm7a; Jhdm1d; BB041802; mKIAA1718; A630082K20Rik; ENSMUSG00000073143
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KDM7A; KDM7A siRNA (Mouse); JHDM1D; KDM7; KIAA1718; Lysine-specific demethylase 7A; JmjC domain-containing histone demethylation protein 1D; Lysine-specific demethylase 7; KDM7A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: KDM7A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 940

• Applicable Applications for KDM7A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse KDM7A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KDM7A sirna

siRNA to inhibit KDM7A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 157909789
• NCBI GeneID: 338523
• NCBI Accession #: NP_001028602.2
• NCBI GenBank Nucleotide #: NM_001033430.4
• UniProt Accession #: Q3UWM4; Q3UWN8; Q6ZPJ5; Q8C969; Q8C9E0; Q91VX8; A6H6E5
• Molecular Weight: 106,075 Da
• NCBI Official Full Name: lysine-specific demethylase 7A
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 7A
• NCBI Official Symbol: Kdm7a
• NCBI Official Synonym Symbols: Jhdm1d; BB041802; mKIAA1718; A630082K20Rik; ENSMUSG00000073143
• NCBI Protein Information: lysine-specific demethylase 7A
• UniProt Protein Name: Lysine-specific demethylase 7A
• Protein Family: Lysine-specific demethylase
• UniProt Gene Name: Kdm7a
• UniProt Synonym Gene Names: Jhdm1d; Kdm7; Kiaa1718
• UniProt Entry Name: KDM7A_MOUSE

Uniprot Description

JHDM1D: Histone demethylase required for brain development. Specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. Demethylates H3K9me2 in absence of H3K4me3. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate. Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.11.-

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: zinc ion binding; histone demethylase activity (H3-K36 specific); metal ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; oxidoreductase activity; histone demethylase activity (H3-K9 specific); methylated histone residue binding; procollagen-proline dioxygenase activity; 2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity; sulfonate dioxygenase activity; dioxygenase activity; iron ion binding; DNA-N1-methyladenine dioxygenase activity

Biological Process: nervous system development; histone H3-K9 demethylation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; chromatin modification