KDM5A sirna

KDM5A siRNA (Mouse)

Cat. Num. AAA827419

• Gene Names: Kdm5a; RBP2; Rbbp2; C76986; Jarid1a; AA409370
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KDM5A; KDM5A siRNA (Mouse); JARID1A; RBP2; Lysine-specific demethylase 5A; Histone demethylase JARID1A; Jumonji/ARID domain-containing protein 1A; Retinoblastoma-binding protein 2; RBBP-2; KDM5A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: KDM5A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1690

• Applicable Applications for KDM5A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse KDM5A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KDM5A sirna

siRNA to inhibit KDM5A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 226958545
• NCBI GeneID: 214899
• NCBI Accession #: NP_666109.2
• NCBI GenBank Nucleotide #: NM_145997.2
• UniProt Accession #: Q3UXZ9; Q3TM94; Q3UMI5; Q66JZ3
• Molecular Weight: 192,216 Da
• NCBI Official Full Name: lysine-specific demethylase 5A
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 5A
• NCBI Official Symbol: Kdm5a
• NCBI Official Synonym Symbols: RBP2; Rbbp2; C76986; Jarid1a; AA409370
• NCBI Protein Information: lysine-specific demethylase 5A
• UniProt Protein Name: Lysine-specific demethylase 5A
• Protein Family: Lysine-specific demethylase
• UniProt Gene Name: Kdm5a
• UniProt Synonym Gene Names: Jarid1a; Rbp2; RBBP-2
• UniProt Entry Name: KDM5A_MOUSE

Uniprot Description

JARID1A: Histone demethylase that specifically demethylates 'Lys- 4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May stimulate transcription mediated by nuclear receptors. May be involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12. Interacts with SUZ12; the interaction is direct. Interacts with the viral protein-binding domain of RB1. Interacts with ESR1, MYC, MYCN and LMO2. Belongs to the JARID1 histone demethylase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; EC 1.14.11.-; Oxidoreductase; Transcription factor; Nuclear receptor co-regulator

Cellular Component: nucleoplasm; cytoplasm; nucleus; cyclin-dependent protein kinase activating kinase holoenzyme complex

Molecular Function: zinc ion binding; metal ion binding; transcription coactivator activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; oxidoreductase activity; procollagen-proline dioxygenase activity; protein binding; 2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity; DNA binding; sulfonate dioxygenase activity; dioxygenase activity; DNA-N1-methyladenine dioxygenase activity; chromatin binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; multicellular organismal development; positive regulation of transcription, DNA-dependent; rhythmic process; circadian regulation of gene expression; chromatin modification