Western Blot (WB)
(Western blot analysis using CK5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.)
Mouse CK5 Monoclonal Antibody | anti-CK5 antibody
CK5 Antibody
WB 1:500-1:2000
IHC 1:200-1:1000
ICC 1:200-1:1000
FCM 1:200-1:400
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Western Blot (WB)
(Western blot analysis using CK5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.)
Western Blot (WB)
(Western blot analysis using CK5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.)
Immunohistochemistry (IHC)
(IHC analysis of human lung cancer tissue, using CK5 Ab at 1:100.)
Immunohistochemistry (IHC)
(IHC analysis of human lung cancer tissue, using CK5 Ab at 1:100.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells using CK5 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells using CK5 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
Uniprot Description
K5: Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP. Belongs to the intermediate filament family.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: cytoplasm; cytosol; intermediate filament; keratin filament; membrane; nucleus
Molecular Function: protein binding; structural constituent of cytoskeleton
Biological Process: epidermis development; hemidesmosome assembly
Disease: Dowling-degos Disease 1; Epidermolysis Bullosa Simplex With Migratory Circinate Erythema; Epidermolysis Bullosa Simplex With Mottled Pigmentation; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Epidermolysis Bullosa Simplex, Dowling-meara Type; Epidermolysis Bullosa Simplex, Generalized; Epidermolysis Bullosa Simplex, Localized
Research Articles on CK5
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Product Notes
The CK5 krt5 (Catalog #AAA9383170) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CK5 Antibody reacts with Human, Mouse, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's CK5 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FCM). ELISA 1:10000 WB 1:500-1:2000 IHC 1:200-1:1000 ICC 1:200-1:1000 FCM 1:200-1:400 IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the CK5 krt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CK5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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