IRAK1 sirna

IRAK1 siRNA (Mouse)

Cat. Num. AAA8230902

• Gene Names: Irak1; IRAK; Plpk; mPLK; IRAK-1; IRAK1b; Il1rak; IRAK1-S; AA408924
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: IRAK1; IRAK1 siRNA (Mouse); IL1RAK; Interleukin-1 receptor-associated kinase 1; IRAK; IRAK-1; Pelle-like protein kinase; mPLK; IRAK1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: IRAK1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 712

• Applicable Applications for IRAK1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse IRAK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IRAK1 sirna

siRNA to inhibit IRAK1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 295789111
• NCBI GeneID: 16179
• NCBI Accession #: NP_001171445.1
• NCBI GenBank Nucleotide #: NM_001177974.1
• UniProt Accession #: Q62406; Q6Y3Z5; Q6Y3Z6; B1AUW4
• Molecular Weight: 77,558 Da
• NCBI Official Full Name: interleukin-1 receptor-associated kinase 1 isoform 4
• NCBI Official Synonym Full Names: interleukin-1 receptor-associated kinase 1
• NCBI Official Symbol: Irak1
• NCBI Official Synonym Symbols: IRAK; Plpk; mPLK; IRAK-1; IRAK1b; Il1rak; IRAK1-S; AA408924
• NCBI Protein Information: interleukin-1 receptor-associated kinase 1
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 1
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: Irak1
• UniProt Synonym Gene Names: Il1rak; IRAK; IRAK-1; mPLK
• UniProt Entry Name: IRAK1_MOUSE

Uniprot Description

IRAK1: a TKL kinase of the IRAK family. Involved in Toll/IL-1 signaling. One of two putative serine/threonine kinases that become associated with the interleukin-1 receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Extensively phosphorylated after its association with IL1-R-1. Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. Partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Three isoforms of the human protein and produced by alternative splicing. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.

Protein type: Protein kinase, TKL; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; TKL group; IRAK family

Cellular Component: interleukin-1 receptor complex; membrane; cytoplasm; lipid particle; nucleus

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; interleukin-1 receptor binding; protein heterodimerization activity; heat shock protein binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: positive regulation of JNK activity; positive regulation of I-kappaB kinase/NF-kappaB cascade; immune system process; protein amino acid autophosphorylation; positive regulation of smooth muscle cell proliferation; cytokine and chemokine mediated signaling pathway; activation of NF-kappaB-inducing kinase; response to lipopolysaccharide; signal transduction; protein amino acid phosphorylation; protein oligomerization; toll-like receptor 2 signaling pathway; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; inhibition of NF-kappaB transcription factor; response to peptidoglycan; lipopolysaccharide-mediated signaling pathway; regulation of cytokine and chemokine mediated signaling pathway; innate immune response; JNK cascade; negative regulation of transcription, DNA-dependent; toll-like receptor 4 signaling pathway; phosphorylation