Rabbit anti-Mouse Irak1 Polyclonal Antibody | anti-Irak1 antibody

Irak1, ID (Irak1, Il1rak, Interleukin-1 receptor-associated kinase 1, Pelle-like protein kinase) (AP)

Cat. Num. AAA6310817

• Gene Names: Irak1; IRAK; Plpk; mPLK; IRAK-1; IRAK1b; Il1rak; IRAK1-S; AA408924
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Irak1; Polyclonal Antibody; ID (Irak1; Il1rak; Interleukin-1 receptor-associated kinase 1; Pelle-like protein kinase) (AP); Pelle-like protein kinase; anti-Irak1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 712

• Applicable Applications for anti-Irak1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Irak1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 404-433 amino acids from the Central region of Mouse Irak1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Irak1 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Irak1 antibody detected the Irak1 protein (arrow).)

Flow Cytometry (FC/FACS)

(Mouse Irak1 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-Irak1 antibody

Irak1 binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. The kinase activity of this enzyme may not be required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.

Product Categories/Family for anti-Irak1 antibody

Antibodies; Protein Kinases

References

Vaughan, T., et al. Mol. Immunol. 47(15):2515-2518(2010). Hoshino, K., et al. J. Immunol. 184(7):3341-3345(2010). Dong, J.W., et al. Am. J. Physiol. Heart Circ. Physiol. 298 (3), H1079-H1087 (2010): Gan, L., et al. Mol. Immunol. 47(6):1278-1282(2010). Maitra, U., et al. J. Biol. Chem. 284(51):35403-35411(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 295789115
• NCBI GeneID: 16179
• NCBI Accession #: NP_001171447.1; NP_032389.2
• NCBI GenBank Nucleotide #: NP_001171447.1
• UniProt Accession #: Q62406
• NCBI Official Full Name: interleukin-1 receptor-associated kinase 1 isoform 2
• NCBI Official Synonym Full Names: interleukin-1 receptor-associated kinase 1
• NCBI Official Symbol: Irak1
• NCBI Official Synonym Symbols: IRAK; Plpk; mPLK; IRAK-1; IRAK1b; Il1rak; IRAK1-S; AA408924
• NCBI Protein Information: interleukin-1 receptor-associated kinase 1
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 1
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: Irak1
• UniProt Synonym Gene Names: Il1rak; IRAK; IRAK-1; mPLK
• UniProt Entry Name: IRAK1_MOUSE

Uniprot Description

IRAK1: a TKL kinase of the IRAK family. Involved in Toll/IL-1 signaling. One of two putative serine/threonine kinases that become associated with the interleukin-1 receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Extensively phosphorylated after its association with IL1-R-1. Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. Partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Three isoforms of the human protein and produced by alternative splicing. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; IRAK family

Cellular Component: cytoplasm; lipid particle; membrane; nucleus

Molecular Function: ATP binding; heat shock protein binding; interleukin-1 receptor binding; kinase activity; nucleotide binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase activity; protein serine/threonine kinase activity; transferase activity

Biological Process: activation of NF-kappaB transcription factor; activation of NF-kappaB-inducing kinase; cytokine and chemokine mediated signaling pathway; immune system process; inhibition of NF-kappaB transcription factor; innate immune response; JNK cascade; lipopolysaccharide-mediated signaling pathway; negative regulation of transcription, DNA-dependent; phosphorylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of JNK activity; positive regulation of MAP kinase activity; positive regulation of smooth muscle cell proliferation; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein oligomerization; regulation of cytokine and chemokine mediated signaling pathway; response to lipopolysaccharide; response to peptidoglycan; signal transduction; toll-like receptor 2 signaling pathway; toll-like receptor 4 signaling pathway