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Typical Testing Data/Standard Curve (for reference only)

Mouse Interleukin 1 Receptor Associated Kinase 1 (IRAK1) RTU ELISA Kit | IRAK1 rtu elisa kit

Mouse Interleukin 1 Receptor Associated Kinase 1 (IRAK1) ELISA Kit

Gene Names
Irak1; IRAK; Plpk; mPLK; IRAK-1; IRAK1b; Il1rak; IRAK1-S; AA408924
Reactivity
Mouse
Synonyms
Interleukin 1 Receptor Associated Kinase 1 (IRAK1); Mouse Interleukin 1 Receptor Associated Kinase 1 (IRAK1) ELISA Kit; IRAK1; IRAK; Pelle; IRAK1 rtu elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Sequence Length
710
Assay Type
Sandwich
Samples
Tissue Homogenates, Cell Lysates or Other Biological Fluids.
Detection Range
0.312-20ng/mL
Sensitivity
0.105ng/mL
RTU ELISA Kit's Advantage
The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IRAK1 rtu elisa kit
Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IRAK1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to IRAK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IRAK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IRAK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
77,558 Da
NCBI Official Full Name
interleukin-1 receptor-associated kinase 1 isoform 4
NCBI Official Synonym Full Names
interleukin-1 receptor-associated kinase 1
NCBI Official Symbol
Irak1
NCBI Official Synonym Symbols
IRAK; Plpk; mPLK; IRAK-1; IRAK1b; Il1rak; IRAK1-S; AA408924
NCBI Protein Information
interleukin-1 receptor-associated kinase 1
UniProt Protein Name
Interleukin-1 receptor-associated kinase 1
UniProt Gene Name
Irak1
UniProt Synonym Gene Names
Il1rak; IRAK; IRAK-1; mPLK

Uniprot Description

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3 ().

Research Articles on IRAK1

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Product Notes

The Mouse IRAK1 irak1 (Catalog #AAA4503730) is a RTU ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA4503730 RTU ELISA Kit recognizes Mouse IRAK1. It is sometimes possible for the material contained within the vial of "Interleukin 1 Receptor Associated Kinase 1 (IRAK1), RTU ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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