INPP5E sirna

INPP5E siRNA (Mouse)

Cat. Num. AAA8211700

• Gene Names: Inpp5e; 72kDa; mKIAA0123; 1200002L24Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: INPP5E; INPP5E siRNA (Mouse); 72 kDa inositol polyphosphate 5-phosphatase; Phosphatidylinositol 4.5-bisphosphate 5-phosphatase; Phosphatidylinositol polyphosphate 5-phosphatase type IV; INPP5E sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: INPP5E siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 605

• Applicable Applications for INPP5E sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse INPP5E gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for INPP5E sirna

siRNA to inhibit INPP5E expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 594190864
• NCBI GeneID: 64436
• NCBI Accession #: NP_001277366.1
• NCBI GenBank Nucleotide #: NM_001290437.1
• UniProt Accession #: Q9JII1; Q3TCC9
• Molecular Weight: 71,915 Da
• NCBI Official Full Name: 72 kDa inositol polyphosphate 5-phosphatase isoform b
• NCBI Official Synonym Full Names: inositol polyphosphate-5-phosphatase E
• NCBI Official Symbol: Inpp5e
• NCBI Official Synonym Symbols: 72kDa; mKIAA0123; 1200002L24Rik
• NCBI Protein Information: 72 kDa inositol polyphosphate 5-phosphatase
• UniProt Protein Name: 72 kDa inositol polyphosphate 5-phosphatase
• Protein Family: 72 kDa inositol polyphosphate 5-phosphatase
• UniProt Gene Name: Inpp5e
• UniProt Entry Name: INP5E_MOUSE

Uniprot Description

INPP5E: Converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2. Specific for lipid substrates, inactive towards water soluble inositol phosphates. Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1). A disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermian hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy and renal disease. Defects in INPP5E are the cause of mental retardation- truncal obesity-retinal dystrophy-micropenis (MORMS). An autosomal recessive disorder characterized by moderate mental retardation, truncal obesity, congenital non-progressive retinal dystrophy, and micropenis in males. The phenotype is similar to Bardet-Biedl syndrome and Cohen syndrome Distinguishing features are the age of onset, the non-progressive nature of the visual impairment, lack of dysmorphic facies, skin or gingival infection, microcephaly, mottled retina, polydactyly, and testicular anomalies. Belongs to the inositol 1,4,5-trisphosphate 5- phosphatase type IV family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Phosphatase (non-protein); Carbohydrate Metabolism - inositol phosphate; EC 3.1.3.36

Cellular Component: Golgi membrane; Golgi apparatus; cytoskeleton; cell projection; membrane; cytoplasm; plasma membrane; axoneme; cilium

Molecular Function: hydrolase activity; phosphoinositide 5-phosphatase activity; inositol-polyphosphate 5-phosphatase activity

Biological Process: dephosphorylation; inositol phosphate dephosphorylation; phosphoinositide dephosphorylation; phosphatidylinositol metabolic process; lipid metabolic process