GJB1 sirna

GJB1 siRNA (Mouse)

Cat. Num. AAA8210352

• Gene Names: Gjb1; Cx32; Cnx32
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GJB1; GJB1 siRNA (Mouse); CXN-32; Gap junction beta-1 protein; Connexin-32; Cx32; GJB1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: GJB1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 283

• Applicable Applications for GJB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GJB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GJB1 sirna

siRNA to inhibit GJB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 699114748
• NCBI GeneID: 14618
• NCBI Accession #: NP_001289425.1
• NCBI GenBank Nucleotide #: NM_001302496.1
• UniProt Accession #: P28230
• Molecular Weight: 32,004 Da
• NCBI Official Full Name: gap junction beta-1 protein
• NCBI Official Synonym Full Names: gap junction protein, beta 1
• NCBI Official Symbol: Gjb1
• NCBI Official Synonym Symbols: Cx32; Cnx32
• NCBI Protein Information: gap junction beta-1 protein
• UniProt Protein Name: Gap junction beta-1 protein
• Protein Family: Gap junction beta-1 protein
• UniProt Gene Name: Gjb1
• UniProt Synonym Gene Names: Cxn-32; Cx32
• UniProt Entry Name: CXB1_MOUSE

NCBI Description

This gene is a member of the gap junction protein (connexin) family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of ions and small molecules between cells. Mutations in a similar gene in human cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Oct 2014]

Uniprot Description

GJB1: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Defects in GJB1 are the cause of Charcot-Marie-Tooth disease X-linked type 1 (CMTX1); also designated CMT- X. CMTX1 is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot- Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies characterized by severely reduced motor nerve conduction velocities (NCVs) (less than 38m/s) and segmental demyelination and remyelination, and primary peripheral axonal neuropathies characterized by normal or mildly reduced NCVs and chronic axonal degeneration and regeneration on nerve biopsy. CMTX1 has both demyelinating and axonal features. Central nervous system involvement may occur. Defects in GJB1 may contribute to the phenotype of Dejerine-Sottas syndrome (DSS); also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS is a severe degenerating neuropathy of the demyelinating Charcot-Marie-Tooth disease category, with onset by age 2 years. DSS is characterized by motor and sensory neuropathy with very slow nerve conduction velocities, increased cerebrospinal fluid protein concentrations, hypertrophic nerve changes, delayed age of walking as well as areflexia. There are both autosomal dominant and autosomal recessive forms of Dejerine- Sottas syndrome. Belongs to the connexin family. Beta-type (group I) subfamily.

Protein type: Membrane protein, integral; Channel, misc.; Motility/polarity/chemotaxis; Membrane protein, multi-pass

Cellular Component: connexon complex; membrane; endoplasmic reticulum; cytoplasm; plasma membrane; integral to membrane; gap junction; cell junction

Molecular Function: protein homodimerization activity; gap junction channel activity

Biological Process: purine ribonucleotide transport; cell communication; transmembrane transport; protein oligomerization