Rabbit anti-Human, Mouse GNAO1 Polyclonal Antibody | anti-GNAO1 antibody

GNAO1, CT (GNAO1, Guanine nucleotide-binding protein G(o) subunit alpha) (Biotin)

Cat. Num. AAA6302910

• Gene Names: GNAO1; GNAO; EIEE17; G-ALPHA-o
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GNAO1; Polyclonal Antibody; CT (GNAO1; Guanine nucleotide-binding protein G(o) subunit alpha) (Biotin); Guanine nucleotide-binding protein G(o) subunit alpha; anti-GNAO1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-GNAO1 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GNAO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 299~328 amino acids from the C-terminal region of human GNAO1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GNAO1 Antibody (C-term) in mouse brain tissue lysates (35ug/lane). GNAO1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of GNAO1 (arrow) using rabbit polyclonal GNAO1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GNAO1 gene.)

Flow Cytometry (FC/FACS)

(GNAO1 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GNAO1 antibody

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(o) protein function is not clear.

Product Categories/Family for anti-GNAO1 antibody

Antibodies; Binding Proteins

References

Yi, F., et.al., J. Biol. Chem. 266 (6), 3900-3906 (1991).

NCBI and Uniprot Product Information

• NCBI GI #: 232133
• NCBI GeneID: 2775
• UniProt Accession #: P09471; P29777; Q8TD72; Q9UMV4
• Molecular Weight: 354
• NCBI Official Full Name: Guanine nucleotide-binding protein G(o) subunit alpha
• NCBI Official Synonym Full Names: guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
• NCBI Official Symbol: GNAO1
• NCBI Official Synonym Symbols: GNAO; EIEE17; G-ALPHA-o
• NCBI Protein Information: guanine nucleotide-binding protein G(o) subunit alpha; guanine nucleotide-binding protein G(o) subunit alpha
• UniProt Protein Name: Guanine nucleotide-binding protein G(o) subunit alpha
• UniProt Gene Name: GNAO1
• UniProt Entry Name: GNAO_HUMAN

NCBI Description

The protein encoded by this gene represents the alpha subunit of the Go heterotrimeric G-protein signal-transducing complex. Defects in this gene are a cause of early-onset epileptic encephalopathy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]

Uniprot Description

G-alpha(o): Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(o) protein function is not clear. Stimulated by RGS14. Interacts with RGS14. G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Belongs to the G-alpha family. G(i/o/t/z) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, heterotrimeric alpha G((i/o/t/z)); G protein; G protein, heterotrimeric

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: neuron projection; plasma membrane; heterotrimeric G-protein complex

Molecular Function: corticotropin-releasing hormone receptor 1 binding; GTPase activity; signal transducer activity; GTP binding; mu-type opioid receptor binding; metal ion binding; G-protein beta/gamma-subunit binding; GTPase activating protein binding; metabotropic serotonin receptor binding

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; response to drug; negative regulation of calcium ion transport; response to hydrogen peroxide; muscle contraction; metabolic process; response to cytokine stimulus; forebrain development; dopamine receptor signaling pathway; response to morphine; locomotory behavior; neurite development; regulation of heart contraction; positive regulation of GTPase activity; aging

Disease: Epileptic Encephalopathy, Early Infantile, 17