G6PC2 sirna

G6PC2 siRNA (Mouse)

Cat. Num. AAA8227987

• Gene Names: G6pc2; IGRP; G6pc-rs
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: G6PC2; G6PC2 siRNA (Mouse); IGRP; Glucose-6-phosphatase 2; G-6-Pase 2; G6Pase 2; Islet-specific glucose-6-phosphatase catalytic subunit-related protein; G6PC2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: G6PC2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 154

• Applicable Applications for G6PC2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse G6PC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for G6PC2 sirna

siRNA to inhibit G6PC2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 578888063
• NCBI GeneID: 14378
• NCBI Accession #: NP_001276785.1
• NCBI GenBank Nucleotide #: NM_001289856.1
• UniProt Accession #: Q9Z186; Q2M2M7; A2AUN2
• Molecular Weight: 17,894 Da
• NCBI Official Full Name: glucose-6-phosphatase 2 isoform 2
• NCBI Official Synonym Full Names: glucose-6-phosphatase, catalytic, 2
• NCBI Official Symbol: G6pc2
• NCBI Official Synonym Symbols: IGRP; G6pc-rs
• NCBI Protein Information: glucose-6-phosphatase 2
• UniProt Protein Name: Glucose-6-phosphatase 2
• Protein Family: Glucose-6-phosphatase
• UniProt Gene Name: G6pc2
• UniProt Synonym Gene Names: Igrp; G-6-Pase 2; G6Pase 2
• UniProt Entry Name: G6PC2_MOUSE

NCBI Description

This gene encodes an enzyme that belongs to the glucose-6-phosphatase catalytic subunit family. Members of this family catalyze the hydrolysis of glucose-6-phosphate, the terminal step in gluconeogenic and glycogenolytic pathways, to release glucose into the bloodstream. The family member encoded by this gene is found specifically in pancreatic islets but has not been shown to have phosphotransferase or phosphatase activity exhibited by a similar liver enzyme. The non-obese diabetic (NOD) mouse is a model for human type 1 diabetes, an autoimmune disease in which T lymphocytes attack and destroy insulin-producing pancreatic beta cells. In NOD mice, the protein encoded by this gene is a major target of cell-mediated autoimmunity. Variations in the human and mouse genes are associated with lower fasting plasma glucose levels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

G6PC2: May hydrolyze glucose-6-phosphate to glucose in the endoplasmic reticulum. May be responsible for glucose production through glycogenolysis and gluconeogenesis. Belongs to the glucose-6-phosphatase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Carbohydrate Metabolism - galactose; Transporter, SLC family; EC 3.1.3.9; Membrane protein, multi-pass; Transporter; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Endoplasmic reticulum; Carbohydrate Metabolism - starch and sucrose; Phosphatase (non-protein)

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane; integral to endoplasmic reticulum membrane

Molecular Function: hydrolase activity; glucose-6-phosphatase activity; catalytic activity

Biological Process: glucose 6-phosphate metabolic process; glucose homeostasis; regulation of insulin secretion; gluconeogenesis