FBXW5 sirna

FBXW5 siRNA (Mouse)

Cat. Num. AAA8220957

• Gene Names: Fbxw5; Fbw5; AI159739
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FBXW5; FBXW5 siRNA (Mouse); FBW5; F-box/WD repeat-containing protein 5; F-box and WD-40 domain-containing protein 5; FBXW5 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: FBXW5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 626

• Applicable Applications for FBXW5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FBXW5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FBXW5 sirna

siRNA to inhibit FBXW5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7305047
• NCBI GeneID: 30839
• NCBI Accession #: NP_038936.1
• NCBI GenBank Nucleotide #: NM_013908.4
• UniProt Accession #: Q9QXW2; Q3U368; A2AJ36; A2AJ37; E9QMP0
• Molecular Weight: 69,687 Da
• NCBI Official Full Name: F-box/WD repeat-containing protein 5
• NCBI Official Synonym Full Names: F-box and WD-40 domain protein 5
• NCBI Official Symbol: Fbxw5
• NCBI Official Synonym Symbols: Fbw5; AI159739
• NCBI Protein Information: F-box/WD repeat-containing protein 5
• UniProt Protein Name: F-box/WD repeat-containing protein 5
• Protein Family: F-box/WD repeat-containing protein
• UniProt Gene Name: Fbxw5
• UniProt Synonym Gene Names: Fbw5
• UniProt Entry Name: FBXW5_MOUSE

Uniprot Description

FBXW5: Substrate recognition component of both SCF (SKP1-CUL1- F-box protein) and DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes. Substrate recognition component of the SCF(FBXW5) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of SASS6 during S phase, leading to prevent centriole reduplication. Substrate-specific adapter of the DCX(FBXW5) E3 ubiquitin-protein ligase complex which mediates the polyubiquitination and subsequent degradation of TSC2. May also act as a negative regulator of MAP3K7/TAK1 signaling in the interleukin-1B (IL1B) signaling pathway. Belongs to the FBXW5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Cellular Component: cytoplasm; SCF ubiquitin ligase complex

Molecular Function: protein binding; protein kinase binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; mitosis; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination; centrosome duplication