Western Blot (WB)
(Western Blot analysis of KIF20A expression in transfected 293T cell line by KIF20A polyclonal antibody. Lane 1: KIF20A transfected lysate (97.9kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human KIF20A Polyclonal Antibody | anti-KIF20A antibody
KIF20A (MKLP2, RAB6KIFL, Kinesin-like Protein KIF20A, GG10_2, Mitotic Kinesin-like Protein 2, Rab6-interacting Kinesin-like Protein, Rabkinesin-6)
Gene Names
KIF20A; MKLP2; RAB6KIFL
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
KIF20A; Polyclonal Antibody; KIF20A (MKLP2; RAB6KIFL; Kinesin-like Protein KIF20A; GG10_2; Mitotic Kinesin-like Protein 2; Rab6-interacting Kinesin-like Protein; Rabkinesin-6); Anti -KIF20A (MKLP2; anti-KIF20A antibody
Host
Mouse
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human KIF20A.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MSQGILSPPAGLLSDDDVVVSPMFESTAADLGSVVRKNLLSDCSVVSTSLEDKQQVPSEDSMEKVKVYLRVRPLLPSELERQEDQGCVRIENVETLVLQAPKDSFALKSNERGIGQATHRFTFSQIFGPEVGQASFFNLTVKEMVKDVLKGQNWLIYTYGVTNSGKTHTIQGTIKDGGILPRSLALIFNSLQGQLHPTPDLKPLLSNEVIWLDSKQIRQEEMKKLSLLNGGLQEEELSTSLKRSVYIESRIGTSTSFDSGIAGLSSISQCTSSSQLDETSHRWAQPDTAPLPVPANIRFSIWISFFEIYNELLYDLLEPPSQQRKRQTLRLCEDQNGNPYVKDLNWIHVQDAEEAWKLLKVGRKNQSFASTHLNQNSSRSHSIFSIRILHLQGEGDIVPKISELSLCDLAGSERCKDQKSGERLKEAGNINTSLHTLGRCIAALRQNQQNRSKQNLVPFRDSKLTRVFQGFFTGRGRSCMIVNVNPCASTYDETLHVAKFSAIASQLVHAPPMQLGFPSLHSFIKEHSLQVSPSLEKGAKADTGLDDDIENEADISMYGKEELLQVVEAMKTLLLKERQEKLQLEMHLRDEICNEMVEQMQQREQWCSEHLDTQKELLEEMYEEKLNILKESLTSFYQEEIQERDEKIEELEALLQEARQQSVAHQQSGSELALRRSQRLAASASTQQLQEVKAKLQQCKAELNSTTEELHKYQKMLEPPPSAKPFTIDVDKKLEEGQKNIRLLRTELQKLGESLQSAERACCHSTGAGKLRQALTTCDDILIKQDQTLAELQNNMVLVKLDLRKKAACIAEQYHTVLKLQGQVSAKKRLGTNQENQQPNQQPPGKKPFLRNLLPRTPTCQSSTDCSPYARILRSRRSPLLKSGPFGKKY
Applicable Applications for anti-KIF20A antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Immunogen
Full length human KIF20A, aa1-890 (NP_005724).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of KIF20A expression in transfected 293T cell line by KIF20A polyclonal antibody. Lane 1: KIF20A transfected lysate (97.9kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of KIF20A expression in transfected 293T cell line by KIF20A polyclonal antibody. Lane 1: KIF20A transfected lysate (97.9kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-KIF20A antibody
KIF20A interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility (By similarity).
Product Categories/Family for anti-KIF20A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
100,278 Da
NCBI Official Full Name
kinesin-like protein KIF20A
NCBI Official Synonym Full Names
kinesin family member 20A
NCBI Official Symbol
KIF20A
NCBI Official Synonym Symbols
MKLP2; RAB6KIFL
NCBI Protein Information
kinesin-like protein KIF20A; GG10_2; rabkinesin-6; mitotic kinesin-like protein 2; rab6-interacting kinesin-like protein; RAB6 interacting, kinesin-like (rabkinesin6)
UniProt Protein Name
Kinesin-like protein KIF20A
Protein Family
UniProt Gene Name
KIF20A
UniProt Synonym Gene Names
MKLP2; RAB6KIFL; MKlp2
UniProt Entry Name
KI20A_HUMAN
Uniprot Description
Function: Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility. Ref.5
Subcellular location: Golgi apparatus
By similarity. Cytoplasm › cytoskeleton › spindle Ref.5.
Post-translational modification: Phosphorylated by PLK1 at Ser-528 during mitosis, creating a docking site for PLK1 and recruiting PLK1 at central spindle. Ref.5
Sequence similarities: Belongs to the kinesin-like protein family.Contains 1 kinesin-motor domain.
Research Articles on KIF20A
Similar Products
Product Notes
The KIF20A kif20a (Catalog #AAA645649) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KIF20A (MKLP2, RAB6KIFL, Kinesin-like Protein KIF20A, GG10_2, Mitotic Kinesin-like Protein 2, Rab6-interacting Kinesin-like Protein, Rabkinesin-6) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KIF20A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the KIF20A kif20a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MSQGILSPPA GLLSDDDVVV SPMFESTAAD LGSVVRKNLL SDCSVVSTSL EDKQQVPSED SMEKVKVYLR VRPLLPSELE RQEDQGCVRI ENVETLVLQA PKDSFALKSN ERGIGQATHR FTFSQIFGPE VGQASFFNLT VKEMVKDVLK GQNWLIYTYG VTNSGKTHTI QGTIKDGGIL PRSLALIFNS LQGQLHPTPD LKPLLSNEVI WLDSKQIRQE EMKKLSLLNG GLQEEELSTS LKRSVYIESR IGTSTSFDSG IAGLSSISQC TSSSQLDETS HRWAQPDTAP LPVPANIRFS IWISFFEIYN ELLYDLLEPP SQQRKRQTLR LCEDQNGNPY VKDLNWIHVQ DAEEAWKLLK VGRKNQSFAS THLNQNSSRS HSIFSIRILH LQGEGDIVPK ISELSLCDLA GSERCKDQKS GERLKEAGNI NTSLHTLGRC IAALRQNQQN RSKQNLVPFR DSKLTRVFQG FFTGRGRSCM IVNVNPCAST YDETLHVAKF SAIASQLVHA PPMQLGFPSL HSFIKEHSLQ VSPSLEKGAK ADTGLDDDIE NEADISMYGK EELLQVVEAM KTLLLKERQE KLQLEMHLRD EICNEMVEQM QQREQWCSEH LDTQKELLEE MYEEKLNILK ESLTSFYQEE IQERDEKIEE LEALLQEARQ QSVAHQQSGS ELALRRSQRL AASASTQQLQ EVKAKLQQCK AELNSTTEEL HKYQKMLEPP PSAKPFTIDV DKKLEEGQKN IRLLRTELQK LGESLQSAER ACCHSTGAGK LRQALTTCDD ILIKQDQTLA ELQNNMVLVK LDLRKKAACI AEQYHTVLKL QGQVSAKKRL GTNQENQQPN QQPPGKKPFL RNLLPRTPTC QSSTDCSPYA RILRSRRSPL LKSGPFGKKY. It is sometimes possible for the material contained within the vial of "KIF20A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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