EZH2 sirna

EZH2 siRNA (Mouse)

Cat. Num. AAA8213694

• Gene Names: Ezh2; KMT6; Enx-1; Enx1h; mKIAA4065
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EZH2; EZH2 siRNA (Mouse); ENX1H; Histone-lysine N-methyltransferase EZH2; ENX-1; Enhancer of zeste homolog 2; EZH2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: EZH2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 746

• Applicable Applications for EZH2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EZH2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EZH2 sirna

siRNA to inhibit EZH2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 226442803
• NCBI GeneID: 14056
• NCBI Accession #: NP_031997.2
• NCBI GenBank Nucleotide #: NM_007971.2
• UniProt Accession #: Q61188; Q99L74; Q9R090
• Molecular Weight: 80,536 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase EZH2 isoform 1
• NCBI Official Synonym Full Names: enhancer of zeste homolog 2 (Drosophila)
• NCBI Official Symbol: Ezh2
• NCBI Official Synonym Symbols: KMT6; Enx-1; Enx1h; mKIAA4065
• NCBI Protein Information: histone-lysine N-methyltransferase EZH2
• UniProt Protein Name: Histone-lysine N-methyltransferase EZH2
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: Ezh2
• UniProt Synonym Gene Names: Enx1h
• UniProt Entry Name: EZH2_MOUSE

Uniprot Description

EZH2: a histone H3 Lys 27 (H3K27) methyltransferase and Polycomb group protein with oncogenic activity. A master regulatory protein that plays a critical role in development. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates K9 and K27 of histone H3, leading to transcriptional repression of the affected target genes. PRC2 includes the Ezh2, EED, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The recruitment of PRC2 to a promoter leads to increased levels of di- and trimethylation of H3K27 (H3K27me2/3), regulating the balance between self-renewal and differentiation of embryonic stem cells (ESCs). The recruitment of PRC2 to promoters in ES cells is interdependent on Jarid 2. ESCs lacking the PRC2 component EED are deficient in Jarid2 promoter activity, while the knockdown of Jarid 2 reduces PRC2 at its target promoters. Able to mono-, di- and trimethylate K27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, p14ARF and retinoic acid target genes. Binds ATRX via the SET domain. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC1 and HDAC2. Interacts with PRAME. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis. Expression decreases during senescence of embryonic fibroblasts. Expression peaks at the G1/S phase boundary. Expression is induced by E2F1, E2F2 and E2F3. Expression is reduced in cells subject to numerous types of stress including UV-, IR- and bleomycin-induced DNA damage and by activation of p53. It has been reported that phosphorylation by AKT1 on S21 reduces methyltransferase activity.

Protein type: Methyltransferase; EC 2.1.1.43; Methyltransferase, protein lysine

Cellular Component: nucleoplasm; pronucleus; ESC/E(Z) complex; cytoplasm; nuclear chromatin; chromosome; nucleus

Molecular Function: transferase activity; methyltransferase activity; histone methyltransferase activity; protein binding; chromatin DNA binding; sequence-specific DNA binding; RNA binding; chromatin binding; histone-lysine N-methyltransferase activity

Biological Process: negative regulation of striated muscle cell differentiation; methylation; regulation of protein amino acid phosphorylation; transcription, DNA-dependent; regulation of gliogenesis; rhythmic process; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; satellite cell compartment self-renewal involved in skeletal muscle regeneration; regulation of circadian rhythm; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; histone methylation; negative regulation of retinoic acid receptor signaling pathway; cerebellar cortex development; positive regulation of MAP kinase activity; regulation of neurogenesis; regulation of transcription, DNA-dependent; negative regulation of gene expression, epigenetic; regulation of gene expression; negative regulation of epidermal cell differentiation; DNA methylation; negative regulation of transcription, DNA-dependent