Western Blot (WB)
(AID antibody (mAb) tested by Western blot. Murine spleen cells were probed with AID antibody at a dilution of 3 ug/ml. Lane 1: Unstimulated spleen cells from wild type mice. Lane 2: Treated (LPS & IL4 for 96 hrs) spleen cells from wild type mice. Lane 3: Unstimulated spleen cells from AID knock-out mice. Lane 4: Treated (LPS & IL4 for 96 hrs) spleen cells from AID knock-out mice.)
Rat anti-Human, Mouse AID Monoclonal Antibody | anti-AID antibody
AID antibody (mAb)(Clone 328.8b)
Gene Names
AICDA; AID; ARP2; CDA2; HIGM2; HEL-S-284
Reactivity
Human, Mouse
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Western Blot
Purity
Protein G Chromatography
Synonyms
AID; Monoclonal Antibody; AID antibody (mAb)(Clone 328.8b); Activation-induced cytidine deaminase; AICDA; ARP2; CDA2; HIGM2; HEL-S-284; anti-AID antibody
Host
Rat
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
328.8b
Purity/Purification
Protein G Chromatography
Form/Format
Purified IgG in 50mM Tris-HCl (pH7.2), 30% glycerol, and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-AID antibody
Chromatin Immunoprecipitation (ChIP), Western Blot (WB)
Application Notes
Applications Validated:
WB: 1-3ug/ml dilution
WB: 1-3ug/ml dilution
Immunogen
This AID antbody was raised against a peptide corresponding to amino acids 178-198 of human AID.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(AID antibody (mAb) tested by Western blot. Murine spleen cells were probed with AID antibody at a dilution of 3 ug/ml. Lane 1: Unstimulated spleen cells from wild type mice. Lane 2: Treated (LPS & IL4 for 96 hrs) spleen cells from wild type mice. Lane 3: Unstimulated spleen cells from AID knock-out mice. Lane 4: Treated (LPS & IL4 for 96 hrs) spleen cells from AID knock-out mice.)
Western Blot (WB)
(AID antibody (mAb) tested by Western blot. Murine spleen cells were probed with AID antibody at a dilution of 3 ug/ml. Lane 1: Unstimulated spleen cells from wild type mice. Lane 2: Treated (LPS & IL4 for 96 hrs) spleen cells from wild type mice. Lane 3: Unstimulated spleen cells from AID knock-out mice. Lane 4: Treated (LPS & IL4 for 96 hrs) spleen cells from AID knock-out mice.)
Related Product Information for anti-AID antibody
Short Description: Our AID antibody (mAb)(Clone 328.8b) was raised in a Rat host. It has been validated for use in Chromatin Immunoprecipitation and Western blot; it has been shown to react with Human and Mouse samples.
Background: AID (Activation-induced cytidine deaminase, AICDA) is a member of the cytidine and deoxycytidylate deaminase family. It acts act as a single-strand DNA deaminase involved in somatic hypermutation, gene conversion and class-switch recombination in developing B-cells and is necessary for efficient antibody responses. It has also been implicated in active DNA demethylation, as deamination of methylcytosine yields thymine, which is read as a mismatch and is replaced by DNA damage repair enzymes.
Background: AID (Activation-induced cytidine deaminase, AICDA) is a member of the cytidine and deoxycytidylate deaminase family. It acts act as a single-strand DNA deaminase involved in somatic hypermutation, gene conversion and class-switch recombination in developing B-cells and is necessary for efficient antibody responses. It has also been implicated in active DNA demethylation, as deamination of methylcytosine yields thymine, which is read as a mismatch and is replaced by DNA damage repair enzymes.
Product Categories/Family for anti-AID antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,614 Da
NCBI Official Full Name
single-stranded DNA cytosine deaminase
NCBI Official Synonym Full Names
activation-induced cytidine deaminase
NCBI Official Symbol
AICDA
NCBI Official Synonym Symbols
AID; ARP2; CDA2; HIGM2; HEL-S-284
NCBI Protein Information
single-stranded DNA cytosine deaminase; cytidine aminohydrolase; epididymis secretory protein Li 284; integrated into Burkitt's lymphoma cell line Ramos
Protein Family
Similar Products
Product Notes
The AID (Catalog #AAA388620) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The AID antibody (mAb)(Clone 328.8b) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AID can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Western Blot (WB). Applications Validated: WB: 1-3ug/ml dilution. Researchers should empirically determine the suitability of the AID for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AID, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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