EXOSC7 sirna

EXOSC7 siRNA (Mouse)

Cat. Num. AAA8240518

• Gene Names: Exosc7; AV212732; mKIAA0116; 2610002K22Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EXOSC7; EXOSC7 siRNA (Mouse); RRP42; Exosome complex exonuclease RRP42; Exosome component 7; Ribosomal RNA-processing protein 42; EXOSC7 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: EXOSC7 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 291

• Applicable Applications for EXOSC7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EXOSC7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EXOSC7 sirna

siRNA to inhibit EXOSC7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 124487127
• NCBI GeneID: 66446
• NCBI Accession #: NP_001074657.1
• NCBI GenBank Nucleotide #: NM_001081188.1
• UniProt Accession #: Q9D0M0; Q4VBW5; Q9CT77
• Molecular Weight: 31,825 Da
• NCBI Official Full Name: exosome complex exonuclease RRP42
• NCBI Official Synonym Full Names: exosome component 7
• NCBI Official Symbol: Exosc7
• NCBI Official Synonym Symbols: AV212732; mKIAA0116; 2610002K22Rik
• NCBI Protein Information: exosome complex exonuclease RRP42
• UniProt Protein Name: Exosome complex exonuclease RRP42
• Protein Family: Exosome complex exonuclease
• UniProt Gene Name: Exosc7
• UniProt Synonym Gene Names: Rrp42
• UniProt Entry Name: EXOS7_MOUSE

Uniprot Description

EXOSC7: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. Belongs to the RNase PH family.

Protein type: EC 3.1.13.-; RNA processing; Ribonuclease; Nucleolus

Cellular Component: cytoplasm; exosome (RNase complex); nucleus

Molecular Function: RNA binding

Biological Process: rRNA processing