ERCC2 sirna

ERCC2 siRNA (Mouse)

Cat. Num. AAA828448

• Gene Names: Ercc2; XPD; CXPD; Ercc-2; AA407812; AU020867; AW240756
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ERCC2; ERCC2 siRNA (Mouse); XPD; TFIIH basal transcription factor complex helicase XPD subunit; CXPD; DNA excision repair protein ERCC-2; DNA repair protein complementing XP-D cells; Xeroderma pigmentosum group D-complementing protein; ERCC2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ERCC2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 760

• Applicable Applications for ERCC2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ERCC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ERCC2 sirna

siRNA to inhibit ERCC2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31542614
• NCBI GeneID: 13871
• NCBI Accession #: NP_031975.2
• NCBI GenBank Nucleotide #: NM_007949.4
• UniProt Accession #: O08811; Q8C487; Q9DC01
• Molecular Weight: 86,842 Da
• NCBI Official Full Name: TFIIH basal transcription factor complex helicase XPD subunit
• NCBI Official Synonym Full Names: excision repair cross-complementing rodent repair deficiency, complementation group 2
• NCBI Official Symbol: Ercc2
• NCBI Official Synonym Symbols: XPD; CXPD; Ercc-2; AA407812; AU020867; AW240756
• NCBI Protein Information: TFIIH basal transcription factor complex helicase XPD subunit
• UniProt Protein Name: TFIIH basal transcription factor complex helicase XPD subunit
• Protein Family: TFIIH basal transcription factor complex helicase
• UniProt Gene Name: Ercc2
• UniProt Synonym Gene Names: Xpd
• UniProt Entry Name: ERCC2_MOUSE

Uniprot Description

ERCC2: ATP-dependent 5'-3' DNA helicase, component of the core- TFIIH basal transcription factor. Involved in nucleotide excision repair (NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase (CAK) complex, composed of CDK7, cyclin H and MAT1, to the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. Might also have a role in aging process and could play a causative role in the generation of skin cancers. Belongs to the helicase family. RAD3/XPD subfamily. One of the six subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor. The interaction with GTF2H2 results in the stimulation of the 5'-->3' helicase activity. Interacts with Epstein-Barr virus EBNA2.

Protein type: EC 3.6.4.12; Helicase

Cellular Component: cytoskeleton; holo TFIIH complex; cytoplasm; spindle; nucleus; cyclin-dependent protein kinase activating kinase holoenzyme complex

Molecular Function: protein C-terminus binding; DNA-dependent ATPase activity; hydrolase activity; metal ion binding; nucleotide binding; iron-sulfur cluster binding; protein N-terminus binding; ATP-dependent helicase activity; helicase activity; protein kinase activity; RNA polymerase subunit kinase activity; ATP-dependent DNA helicase activity; DNA binding; nucleic acid binding; 4 iron, 4 sulfur cluster binding; 5'-3' DNA helicase activity; ATP binding; hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides

Biological Process: skin development; transcription from RNA polymerase II promoter; extracellular matrix organization and biogenesis; erythrocyte maturation; apoptosis; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; embryonic cleavage; bone mineralization; chromosome segregation; post-embryonic development; hair cycle process; regulation of transcription, DNA-dependent; UV protection; transcription-coupled nucleotide-excision repair; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; response to UV; aging; positive regulation of DNA binding; transcription, DNA-dependent; in utero embryonic development; multicellular organism growth; nucleotide-excision repair, DNA incision; hair cell differentiation; hair follicle maturation; DNA repair; DNA duplex unwinding; cell proliferation; myelin formation in the central nervous system; spinal cord development; nucleotide-excision repair; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; response to DNA damage stimulus