Rabbit anti-Human ERCC2 Polyclonal Antibody | anti-ERCC2 antibody

ERCC2 Rabbit Polyclonal

Cat. Num. AAA769065

• Gene Names: ERCC2; EM9; TTD; XPD; TTD1; COFS2; TFIIH
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: ERCC2; Polyclonal Antibody; ERCC2 Rabbit Polyclonal; anti-ERCC2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 760

• Applicable Applications for anti-ERCC2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Immunogen: Excision repair cross-complementing rodent repair deficiency, complementation group 2
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lymphoma using MBS769065 (ERCC2 antibody) at dilution of 1:100)

Western Blot (WB)

(HeLa cells were subjected to SDS PAGE followed by western blot with MBS769065 (ERCC2 antibody) at dilution of 1:500)

NCBI and Uniprot Product Information

• NCBI GI #: 17939382
• NCBI GeneID: 2068
• Molecular Weight: 46,274 Da
• NCBI Official Full Name: ERCC2
• NCBI Official Synonym Full Names: ERCC excision repair 2, TFIIH core complex helicase subunit
• NCBI Official Symbol: ERCC2
• NCBI Official Synonym Symbols: EM9; TTD; XPD; TTD1; COFS2; TFIIH
• NCBI Protein Information: TFIIH basal transcription factor complex helicase XPD subunit
• UniProt Protein Name: TFIIH basal transcription factor complex helicase XPD subunit
• Protein Family: TFIIH basal transcription factor complex helicase
• UniProt Gene Name: ERCC2
• UniProt Synonym Gene Names: XPD; XPDC; BTF2 p80; TFIIH 80 kDa subunit; TFIIH p80
• UniProt Entry Name: ERCC2_HUMAN

NCBI Description

The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

ERCC2: ATP-dependent 5'-3' DNA helicase, component of the core- TFIIH basal transcription factor. Involved in nucleotide excision repair (NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase (CAK) complex, composed of CDK7, cyclin H and MAT1, to the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. Might also have a role in aging process and could play a causative role in the generation of skin cancers. Belongs to the helicase family. RAD3/XPD subfamily. One of the six subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor. The interaction with GTF2H2 results in the stimulation of the 5'-->3' helicase activity. Interacts with Epstein-Barr virus EBNA2.

Protein type: EC 3.6.4.12; Helicase

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: cyclin-dependent protein kinase activating kinase holoenzyme complex; cytoplasm; holo TFIIH complex; nucleoplasm; nucleus; spindle

Molecular Function: 4 iron, 4 sulfur cluster binding; 5'-3' DNA helicase activity; ATP binding; ATP-dependent DNA helicase activity; DNA binding; DNA-dependent ATPase activity; metal ion binding; protein binding; protein C-terminus binding; protein kinase activity; protein N-terminus binding; RNA polymerase subunit kinase activity

Biological Process: aging; apoptosis; bone mineralization; cell proliferation; chromosome segregation; embryonic cleavage; erythrocyte maturation; extracellular matrix organization and biogenesis; hair cell differentiation; hair follicle maturation; in utero embryonic development; mRNA capping; multicellular organism growth; myelin formation in the central nervous system; nucleotide-excision repair; nucleotide-excision repair, DNA duplex unwinding; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 3'-to lesion; nucleotide-excision repair, DNA incision, 5'-to lesion; nucleotide-excision repair, preincision complex assembly; nucleotide-excision repair, preincision complex stabilization; positive regulation of DNA binding; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; post-embryonic development; protein amino acid phosphorylation; response to hypoxia; response to oxidative stress; RNA elongation from RNA polymerase I promoter; RNA elongation from RNA polymerase II promoter; spinal cord development; termination of RNA polymerase I transcription; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase I promoter; transcription initiation from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair; UV protection; viral reproduction

Disease: Cerebrooculofacioskeletal Syndrome 2; Trichothiodystrophy, Photosensitive; Xeroderma Pigmentosum, Complementation Group D