EIF2AK2 sirna

EIF2AK2 siRNA (Mouse)

Cat. Num. AAA8205103

• Gene Names: Eif2ak2; Pkr; Tik; Prkr; AI467567; AI747578; 2310047A08Rik; 4732414G15Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EIF2AK2; EIF2AK2 siRNA (Mouse); PKR; PRKR; TIK; Interferon-induced. double-stranded RNA-activated protein kinase; Eukaryotic translation initiation factor 2-alpha kinase 2; eIF-2A protein kinase 2; Interferon-inducible RNA-dependent protein kinase; P1/eIF-2A protein kinase; Protein kinase RNA-activated; Serine/threonine-protein kinase TIK; Tyrosine-protein kinase EIF2AK2; p68 kinase; EIF2AK2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: EIF2AK2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 515

• Applicable Applications for EIF2AK2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EIF2AK2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EIF2AK2 sirna

siRNA to inhibit EIF2AK2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6755160
• NCBI GeneID: 19106
• NCBI Accession #: NP_035293.1
• NCBI GenBank Nucleotide #: NM_011163.4
• UniProt Accession #: Q03963; Q61742; Q62026
• Molecular Weight: 58,280 Da
• NCBI Official Full Name: interferon-induced, double-stranded RNA-activated protein kinase
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2-alpha kinase 2
• NCBI Official Symbol: Eif2ak2
• NCBI Official Synonym Symbols: Pkr; Tik; Prkr; AI467567; AI747578; 2310047A08Rik; 4732414G15Rik
• NCBI Protein Information: interferon-induced, double-stranded RNA-activated protein kinase
• UniProt Protein Name: Interferon-induced, double-stranded RNA-activated protein kinase
• Protein Family: Interferon-induced, double-stranded RNA-activated protein kinase
• UniProt Gene Name: Eif2ak2
• UniProt Synonym Gene Names: Pkr; Prkr; Tik; eIF-2A protein kinase 2; PKR; Protein kinase R
• UniProt Entry Name: E2AK2_MOUSE

Uniprot Description

PKR: a protein kinase of the PEK family. Upon binding double-stranded RNA, it becomes autophosphorylated and activated. Phosphorylates and inhibits the alpha subunit of eIF2 alpha, which leads to an inhibition of the initiation of protein synthesis. Controls the activation of several transcription factors such as NF-kappaB, p53 and Stats. Mediates apoptosis induced by many different stimuli, such as LPS, TNF-alpha, viral infection and serum starvation.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Translation; EC 2.7.10.2; EC 2.7.11.1; Protein kinase, Other; Other group; PEK family

Cellular Component: membrane; perinuclear region of cytoplasm; cytoplasm; nucleus

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; RNA binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; double-stranded RNA binding; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: positive regulation of cytokine production; translation; transcription, DNA-dependent; activation of MAPKK activity; positive regulation of apoptosis; unfolded protein response; immune system process; response to toxin; protein amino acid autophosphorylation; response to virus; response to lipopolysaccharide; protein amino acid phosphorylation; positive regulation of stress-activated MAPK cascade; positive regulation of chemokine production; activation of NF-kappaB transcription factor; response to vitamin E; regulation of transcription, DNA-dependent; negative regulation of viral genome replication; negative regulation of translation; innate immune response; negative regulation of osteoblast proliferation; defense response to virus; phosphorylation; negative regulation of apoptosis