Human Interferon-induced, double-stranded RNA-activated protein kinase ELISA Kit | EIF2AK2 elisa kit

Human Interferon-induced, double-stranded RNA-activated protein kinase ELISA Kit

Cat. Num. AAA760701

• Gene Names: Eif2ak2; Pkr; Tik; Prkr; AI467567; AI747578; 2310047A08Rik; 4732414G15Rik
• Reactivity: Human
• Synonyms: Interferon-induced; double-stranded RNA-activated protein kinase; Human Interferon-induced; double-stranded RNA-activated protein kinase ELISA Kit; EIF2AK2/PRKR/PRKRA/PKR/EIF2AK1/p68 kinase/double stranded RNA activated protein kinase/eIF-2A protein kinase 2/eukaryotic translation initiation factor 2-alpha kinase 2/P1/eIF-2A protein kinase/interferon-inducible double stranded RNA dependent/interferon-inducible elF2alpha kinase/Interferon-inducible RNA-dependent protein kinase/Protein kinase RNA-activated; EIF2AK2 elisa kit

• Reactivity: Human
• Sequence Length: 515

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for EIF2AK2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human EIF2AK2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human EIF2AK2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EIF2AK2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human EIF2AK2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 6755160
• NCBI GeneID: 19106
• NCBI Accession #: NP_035293.1
• NCBI GenBank Nucleotide #: NM_011163.4
• Molecular Weight: 58,280 Da
• NCBI Official Full Name: interferon-induced, double-stranded RNA-activated protein kinase
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2-alpha kinase 2
• NCBI Official Symbol: Eif2ak2
• NCBI Official Synonym Symbols: Pkr; Tik; Prkr; AI467567; AI747578; 2310047A08Rik; 4732414G15Rik
• NCBI Protein Information: interferon-induced, double-stranded RNA-activated protein kinase
• UniProt Protein Name: Interferon-induced, double-stranded RNA-activated protein kinase
• Protein Family: Interferon-induced, double-stranded RNA-activated protein kinase
• UniProt Gene Name: Eif2ak2
• UniProt Synonym Gene Names: Pkr; Prkr; Tik; eIF-2A protein kinase 2; PKR; Protein kinase R
• UniProt Entry Name: E2AK2_MOUSE

Uniprot Description

PKR: a protein kinase of the PEK family. Upon binding double-stranded RNA, it becomes autophosphorylated and activated. Phosphorylates and inhibits the alpha subunit of eIF2 alpha, which leads to an inhibition of the initiation of protein synthesis. Controls the activation of several transcription factors such as NF-kappaB, p53 and Stats. Mediates apoptosis induced by many different stimuli, such as LPS, TNF-alpha, viral infection and serum starvation.

Protein type: EC 2.7.11.1; EC 2.7.10.2; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, Other; Translation; Other group; PEK family

Cellular Component: cytoplasm; membrane; nucleus; perinuclear region of cytoplasm

Molecular Function: ATP binding; double-stranded RNA binding; eukaryotic translation initiation factor 2alpha kinase activity; kinase activity; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein-tyrosine kinase activity; RNA binding; transferase activity

Biological Process: activation of MAPKK activity; activation of NF-kappaB transcription factor; cellular response to amino acid starvation; defense response to virus; immune system process; innate immune response; negative regulation of apoptosis; negative regulation of osteoblast proliferation; negative regulation of translation; negative regulation of viral genome replication; phosphorylation; positive regulation of apoptosis; positive regulation of chemokine production; positive regulation of cytokine production; positive regulation of stress-activated MAPK cascade; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of transcription, DNA-dependent; response to lipopolysaccharide; response to toxin; response to virus; response to vitamin E; transcription, DNA-dependent; translation; unfolded protein response