EGLN3 sirna

EGLN3 siRNA (Mouse)

Cat. Num. AAA8205625

• Gene Names: Egln3; Phd3; SM-20; AI505553; AI648162; Hif-p4h-3; 2610021G09Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EGLN3; EGLN3 siRNA (Mouse); Egl nine homolog 3; Hypoxia-inducible factor prolyl hydroxylase 3; HIF-PH3; HIF-prolyl hydroxylase 3; HPH-3; Prolyl hydroxylase domain-containing protein 3; PHD3; SM-20; EGLN3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: EGLN3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 239

• Applicable Applications for EGLN3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EGLN3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EGLN3 sirna

siRNA to inhibit EGLN3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 269308213
• NCBI GeneID: 112407
• NCBI Accession #: NP_082409.2
• NCBI GenBank Nucleotide #: NM_028133.2
• UniProt Accession #: Q91UZ4; Q3TCG8; Q8C8M6; Q8CCA8; Q8R5C7
• Molecular Weight: 27,302 Da
• NCBI Official Full Name: egl nine homolog 3
• NCBI Official Synonym Full Names: egl-9 family hypoxia-inducible factor 3
• NCBI Official Symbol: Egln3
• NCBI Official Synonym Symbols: Phd3; SM-20; AI505553; AI648162; Hif-p4h-3; 2610021G09Rik
• NCBI Protein Information: egl nine homolog 3
• UniProt Protein Name: Egl nine homolog 3
• Protein Family: Egl nine
• UniProt Gene Name: Egln3
• UniProt Synonym Gene Names: HIF-PH3; HIF-prolyl hydroxylase 3; HPH-3; PHD3
• UniProt Entry Name: EGLN3_MOUSE

Uniprot Description

EGLN3: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation.

Protein type: Oxidoreductase; EC 1.14.11.29

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: peptidyl-proline dioxygenase activity; dioxygenase activity; L-ascorbic acid binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; metal ion binding; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; oxidoreductase activity; peptidyl-proline 4-dioxygenase activity

Biological Process: apoptosis; regulation of neuron apoptosis; response to hypoxia; peptidyl-proline hydroxylation; protein amino acid hydroxylation; peptidyl-proline hydroxylation to 4-hydroxy-L-proline; response to DNA damage stimulus; regulation of cell proliferation