DNMT3L sirna

DNMT3L siRNA (Mouse)

Cat. Num. AAA8202980

• Gene Names: Dnmt3l; ecat7; D6Ertd14e
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DNMT3L; DNMT3L siRNA (Mouse); DNA; cytosine-5)-methyltransferase 3-like; DNMT3L sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DNMT3L siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 421

• Applicable Applications for DNMT3L sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DNMT3L gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DNMT3L sirna

siRNA to inhibit DNMT3L expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 126158899
• NCBI GeneID: 54427
• NCBI Accession #: NP_001075164.1
• NCBI GenBank Nucleotide #: NM_001081695.2
• UniProt Accession #: Q9CWR8
• Molecular Weight: 47,993 Da
• NCBI Official Full Name: DNA (cytosine-5)-methyltransferase 3-like isoform 1
• NCBI Official Synonym Full Names: DNA (cytosine-5-)-methyltransferase 3-like
• NCBI Official Symbol: Dnmt3l
• NCBI Official Synonym Symbols: ecat7; D6Ertd14e
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 3-like
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 3-like
• Protein Family: DNA (cytosine-5)-methyltransferase
• UniProt Gene Name: Dnmt3l
• UniProt Entry Name: DNM3L_MOUSE

Uniprot Description

DNMT3L: Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3. Belongs to the C5-methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Amino Acid Metabolism - cysteine and methionine

Cellular Component: condensed nuclear chromosome; cytoplasm; nuclear heterochromatin; cytosol; nucleus

Molecular Function: protein binding; enzyme binding; metal ion binding; enzyme activator activity

Biological Process: methylation; positive regulation of catalytic activity; in utero embryonic development; negative regulation of transcription, DNA-dependent; placenta development