DNAJB1 sirna

DNAJB1 siRNA (Mouse)

Cat. Num. AAA8234831

• Gene Names: Dnajb1; DjB1; Hdj1; HSPF1; Hsp40; 0610007I11Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DNAJB1; DNAJB1 siRNA (Mouse); HSP40; HSPF1; DnaJ homolog subfamily B member 1; Heat shock 40 kDa protein 1; Heat shock protein 40; DNAJB1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DNAJB1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 340

• Applicable Applications for DNAJB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DNAJB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DNAJB1 sirna

siRNA to inhibit DNAJB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 9055242
• NCBI GeneID: 81489
• NCBI Accession #: NP_061278.1
• NCBI GenBank Nucleotide #: NM_018808.3
• UniProt Accession #: Q9QYJ3
• Molecular Weight: 38,167 Da
• NCBI Official Full Name: dnaJ homolog subfamily B member 1 isoform 1
• NCBI Official Synonym Full Names: DnaJ (Hsp40) homolog, subfamily B, member 1
• NCBI Official Symbol: Dnajb1
• NCBI Official Synonym Symbols: DjB1; Hdj1; HSPF1; Hsp40; 0610007I11Rik
• NCBI Protein Information: dnaJ homolog subfamily B member 1
• UniProt Protein Name: DnaJ homolog subfamily B member 1
• Protein Family: DnaJ homolog subfamily
• UniProt Gene Name: Dnajb1
• UniProt Synonym Gene Names: Hsp40; Hspf1; HSP40; Heat shock protein 40
• UniProt Entry Name: DNJB1_MOUSE

NCBI Description

This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. The encoded protein may also inhibit apoptosis. Peritoneal macrophages derived from homozygous knockout mice for this gene exhibit impaired heat tolerance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

HSP40: a chaperone protein that translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Interacts with DNAJC3. Interacts with SRPK1.

Protein type: Chaperone; Nucleolus

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: ATPase activator activity; chaperone binding; unfolded protein binding; Hsp70 protein binding; ATPase binding

Biological Process: chaperone cofactor-dependent protein folding; protein folding; positive regulation of ATPase activity