Mouse DnaJ homolog subfamily B member 1 ELISA Kit | Dnajb1 elisa kit

Mouse DnaJ homolog subfamily B member 1 ELISA Kit

Cat. Num. AAA288042

• Gene Names: Dnajb1; DjB1; Hdj1; HSPF1; Hsp40; 0610007I11Rik
• Reactivity: Mouse
• Synonyms: DnaJ homolog subfamily B member 1; Mouse DnaJ homolog subfamily B member 1 ELISA Kit; Dnajb1 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse DnaJ homolog subfamily B member 1. No significant cross-reactivity or interference was observed.
• Sequence Length: 340

• Assay Type: Sandwich
• Detection Range: 0.156-10 ng/ml
• Sensitivity: 0.091 ng/mL
• Intra-Assay CV: <=8.2%
• Inter-Assay CV: <=10.0%
• Recovery: 93%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Dnajb1 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DnaJ homolog subfamily B member 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DnaJ homolog subfamily B member 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DnaJ homolog subfamily B member 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of DnaJ homolog subfamily B member 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 9055242
• NCBI GeneID: 81489
• NCBI Accession #: NP_061278.1
• NCBI GenBank Nucleotide #: NM_018808.3
• UniProt Accession #: Q9QYJ3
• Molecular Weight: 38,167 Da
• NCBI Official Full Name: dnaJ homolog subfamily B member 1 isoform 1
• NCBI Official Synonym Full Names: DnaJ heat shock protein family (Hsp40) member B1
• NCBI Official Symbol: Dnajb1
• NCBI Official Synonym Symbols: DjB1; Hdj1; HSPF1; Hsp40; 0610007I11Rik
• NCBI Protein Information: dnaJ homolog subfamily B member 1
• UniProt Protein Name: DnaJ homolog subfamily B member 1
• Protein Family: DnaJ homolog subfamily
• UniProt Gene Name: Dnajb1
• UniProt Synonym Gene Names: Hsp40; Hspf1; HSP40; Heat shock protein 40
• UniProt Entry Name: DNJB1_MOUSE

NCBI Description

This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. The encoded protein may also inhibit apoptosis. Peritoneal macrophages derived from homozygous knockout mice for this gene exhibit impaired heat tolerance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

HSP40: a chaperone protein that translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Interacts with DNAJC3. Interacts with SRPK1.

Protein type: Chaperone; Nucleolus

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: ATPase activator activity; ATPase binding; chaperone binding; Hsp70 protein binding; unfolded protein binding

Biological Process: chaperone cofactor-dependent protein folding; positive regulation of ATPase activity; protein folding