DMBT1 sirna

DMBT1 siRNA (Mouse)

Cat. Num. AAA8201956

• Gene Names: Dmbt1; CRP; p80; Crpd; DBMT1; gp300; CRP-[a]; CRP-[b]
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DMBT1; DMBT1 siRNA (Mouse); CRPD; Deleted in malignant brain tumors 1 protein; Apactin; CRP-ductin; Glycoprotein 300; gp300; Hensin; Mucin-like glycoprotein; Muclin; Vomeroglandin; p80; DMBT1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DMBT1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1946

• Applicable Applications for DMBT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DMBT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DMBT1 sirna

siRNA to inhibit DMBT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 87239967
• NCBI GeneID: 12945
• NCBI Accession #: NP_031795.2
• NCBI GenBank Nucleotide #: NM_007769.2
• UniProt Accession #: Q60997; Q80YC6; Q9JMJ9
• Molecular Weight: 212,209 Da
• NCBI Official Full Name: deleted in malignant brain tumors 1 protein
• NCBI Official Synonym Full Names: deleted in malignant brain tumors 1
• NCBI Official Symbol: Dmbt1
• NCBI Official Synonym Symbols: CRP; p80; Crpd; DBMT1; gp300; CRP-[a]; CRP-[b]
• NCBI Protein Information: deleted in malignant brain tumors 1 protein
• UniProt Protein Name: Deleted in malignant brain tumors 1 protein
• Protein Family: Deleted in malignant brain tumors 1 protein
• UniProt Gene Name: Dmbt1
• UniProt Synonym Gene Names: Crpd; gp300; Muclin
• UniProt Entry Name: DMBT1_MOUSE

Uniprot Description

DMBT1: May be considered as a candidate tumor suppressor gene for brain, lung, esophageal, gastric, and colorectal cancers. May play roles in mucosal defense system, cellular immune defense and epithelial differentiation. May play a role as an opsonin receptor for SFTPD and SPAR in macrophage tissues throughout the body, including epithelial cells lining the gastrointestinal tract. May play a role in liver regeneration. May be an important factor in fate decision and differentiation of transit-amplifying ductular (oval) cells within the hepatic lineage. Required for terminal differentiation of columnar epithelial cells during early embryogenesis. May function as a binding protein in saliva for the regulation of taste sensation. Binds to HIV-1 envelope protein and has been shown to both inhibit and facilitate viral transmission. Displays a broad calcium-dependent binding spectrum against both Gram-positive and Gram-negative bacteria, suggesting a role in defense against bacterial pathogens. Binds to a range of poly- sulfated and poly-phosphorylated ligands which may explain its broad bacterial-binding specificity. Inhibits cytoinvasion of S.enterica. Associates with the actin cytoskeleton and is involved in its remodeling during regulated exocytosis. Interacts with pancreatic zymogens in a pH-dependent manner and may act as a Golgi cargo receptor in the regulated secretory pathway of the pancreatic acinar cell. Defects in DMBT1 are involved in the development of glioma (GLM). Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Homozygous deletions may be the predominant mechanism of DMBT1 inactivation playing a role in carcinogenesis. DMBT1 is deleted in medulloblastoma and glioblastoma cell lines; point mutations have also been reported in patients with glioma. A loss or reduction of DMBT1 expression has been seen in esophageal, gastric, lung and colorectal carcinomas as well. Belongs to the DMBT1 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Tumor suppressor; Membrane protein, integral; Secreted; Oncoprotein

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; phagocytic vesicle membrane; zymogen granule membrane; extrinsic to membrane; membrane; cytoplasm; integral to membrane; extracellular region; cytoplasmic vesicle; intracellular

Molecular Function: protein binding; scavenger receptor activity

Biological Process: blastocyst development; protein transport; transport; multicellular organismal development; positive regulation of epithelial cell differentiation; cell differentiation; inner cell mass cell proliferation