DLC1 sirna

DLC1 siRNA (Mouse)

Cat. Num. AAA8202226

• Gene Names: Dlc1; HP; dlc-1; Arhgap7; STARD12; A730069N07Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DLC1; DLC1 siRNA (Mouse); ARHGAP7; STARD12; Rho GTPase-activating protein 7; Deleted in liver cancer 1 protein homolog; DLC-1; Rho-type GTPase-activating protein 7; START domain-containing protein 12; StARD12; StAR-related lipid transfer protein 12; DLC1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DLC1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1126

• Applicable Applications for DLC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DLC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DLC1 sirna

siRNA to inhibit DLC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 302699225
• NCBI GeneID: 50768
• NCBI Accession #: NP_001181870.1
• NCBI GenBank Nucleotide #: NM_001194941.1
• UniProt Accession #: Q9R0Z9; Q3TRX3; Q3UH75; Q8R541
• Molecular Weight: 127,257 Da
• NCBI Official Full Name: rho GTPase-activating protein 7 isoform 3
• NCBI Official Synonym Full Names: deleted in liver cancer 1
• NCBI Official Symbol: Dlc1
• NCBI Official Synonym Symbols: HP; dlc-1; Arhgap7; STARD12; A730069N07Rik
• NCBI Protein Information: rho GTPase-activating protein 7
• UniProt Protein Name: Rho GTPase-activating protein 7
• Protein Family: Dynein
• UniProt Gene Name: Dlc1
• UniProt Synonym Gene Names: Arhgap7; Stard12; DLC-1; StARD12
• UniProt Entry Name: RHG07_MOUSE

Uniprot Description

DLC1: Functions as a GTPase-activating protein specific for Rho and an activator of PLCD1 in vivo and induces morphological changes and detachment through cytoskeletal reorganization. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs; Tumor suppressor; GAPs, Rac/Rho

Cellular Component: cortical actin cytoskeleton; focal adhesion; membrane; cytoplasm; stress fiber; actin filament; intracellular; caveola; nucleus; cell junction; cytosol; lipid raft

Molecular Function: phospholipase binding; SH2 domain binding; lipid binding; vinculin binding

Biological Process: heart morphogenesis; focal adhesion formation; caspase activation; hindbrain morphogenesis; negative regulation of stress fiber formation; apoptosis; negative regulation of focal adhesion formation; cytoskeleton organization and biogenesis; signal transduction; positive regulation of protein amino acid dephosphorylation; negative regulation of cell proliferation; regulation of cell shape; negative regulation of Rho protein signal transduction; phospholipase C activation; regulation of actin cytoskeleton organization and biogenesis; forebrain development; neural tube closure; regulation of Rho protein signal transduction; actin cytoskeleton organization and biogenesis; negative regulation of cell migration