DEAF1 sirna

DEAF1 siRNA (Mouse)

Cat. Num. AAA8234090

• Gene Names: Deaf1; NUDR; AU042387; C230009B13Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DEAF1; DEAF1 siRNA (Mouse); Deformed epidermal autoregulatory factor 1 homolog; Nuclear DEAF-1-related transcriptional regulator; NUDR; DEAF1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DEAF1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 566

• Applicable Applications for DEAF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DEAF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DEAF1 sirna

siRNA to inhibit DEAF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 8393248
• NCBI GeneID: 54006
• NCBI Accession #: NP_058570.1
• NCBI GenBank Nucleotide #: NM_016874.3
• UniProt Accession #: Q9Z1T5; Q3UJA4; C7SHZ9
• Molecular Weight: 30,737 Da
• NCBI Official Full Name: deformed epidermal autoregulatory factor 1 homolog isoform 1
• NCBI Official Synonym Full Names: deformed epidermal autoregulatory factor 1 (Drosophila)
• NCBI Official Symbol: Deaf1
• NCBI Official Synonym Symbols: NUDR; AU042387; C230009B13Rik
• NCBI Protein Information: deformed epidermal autoregulatory factor 1 homolog
• UniProt Protein Name: Deformed epidermal autoregulatory factor 1 homolog
• Protein Family: Deformed epidermal autoregulatory factor
• UniProt Gene Name: Deaf1
• UniProt Synonym Gene Names: NUDR
• UniProt Entry Name: DEAF1_MOUSE

Uniprot Description

DEAF1: a transcription factor that binds to its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE). Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction. When secreted, behaves as an inhibitor of cell proliferation, by arresting cells in the G0 or G1 phase.

Protein type: Motility/polarity/chemotaxis; Transcription, coactivator/corepressor

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleolus; nucleus

Molecular Function: DNA binding; metal ion binding

Biological Process: transcription from RNA polymerase II promoter; nervous system development; behavioral fear response; transcription, DNA-dependent; embryonic skeletal development; multicellular organismal development; positive regulation of transcription, DNA-dependent; regulation of mammary gland epithelial cell proliferation; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; neural tube closure; visual learning; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent