Human HLA-DQB2 (HLA class II histocompatibility antigen, DQ beta 2 chain) ELISA Kit | HLA-DQB2 elisa kit

Human HLA-DQB2 (HLA class II histocompatibility antigen, DQ beta 2 chain) ELISA Kit

Cat. Num. AAA7606225

• Gene Names: HLA-DQB2; HLA-DXB; HLA-DQB1
• Reactivity: Human
• Synonyms: HLA-DQB2 (HLA class II histocompatibility antigen; DQ beta 2 chain); Human HLA-DQB2 (HLA class II histocompatibility antigen; DQ beta 2 chain) ELISA Kit; HLA-DQB2 elisa kit

• Reactivity: Human
• Sequence Length: 227

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HLA-DQB2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- HLA-DQB2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- HLA-DQB2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HLA-DQB2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HLA-DQB2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 311893321
• NCBI GeneID: 3120
• NCBI Accession #: NP_001185787.1
• NCBI GenBank Nucleotide #: NM_001198858.1
• UniProt Accession #: P05538; Q29826; Q29870; Q29871; Q29872; Q29873; Q5SR06; A6NIA5
• Molecular Weight: 26,031 Da
• NCBI Official Full Name: HLA class II histocompatibility antigen, DQ beta 2 chain isoform 2
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DQ beta 2
• NCBI Official Symbol: HLA-DQB2
• NCBI Official Synonym Symbols: HLA-DXB; HLA-DQB1
• NCBI Protein Information: HLA class II histocompatibility antigen, DQ beta 2 chain
• UniProt Protein Name: HLA class II histocompatibility antigen, DQ beta 2 chain
• Protein Family: HLA class II histocompatibility antigen
• UniProt Gene Name: HLA-DQB2
• UniProt Synonym Gene Names: HLA-DXB

NCBI Description

HLA-DQB2 belongs to the family of HLA class II beta chain paralogs. Class II molecules are heterodimers consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. They play a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). Polymorphisms in the alpha and beta chains specify the peptide binding specificity, and typing for these polymorphisms is routinely done for bone marrow transplantation. However this gene, HLA-DQB2, is not routinely typed, as it is not thought to have an effect on transplantation. There is conflicting evidence in the literature and public sequence databases for the protein-coding capacity of HLA-DQB2. Because there is evidence of transcription and an intact ORF, HLA-DQB2 is represented in Entrez Gene and in RefSeq as a protein-coding locus. [provided by RefSeq, Oct 2010]

Uniprot Description

HLA-DQB2: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. Belongs to the MHC class II family. Heterodimer of an alpha and a beta subunit; also referred as MHC class II molecule. Dimer formation with HLA-DQA2, but not with HLA-DQA1, is required for efficient exit from the endoplasmic reticulum (ER). In the ER, forms a heterononamer; 3 MHC class II molecules bind to a CD74 homotrimer (also known as invariant chain or HLA class II histocompatibility antigen gamma chain). In the endosomal/lysosomal system; CD74 undergoes sequential degradation by various proteases; leaving a small fragment termed CLIP on each MHC class II molecule. MHC class II molecule interacts with HLA_DM, and HLA_DO in B-cells, in order to release CLIP and facilitate the binding of antigenic peptides. Association with HLA-DMA also occurs in skin Langerhans cells, in post-Golgi compartments. 2 isoforms of the human protein are produced by alternative splicing

Chromosomal Location of Human Ortholog: 6p21.32

Cellular Component: Golgi membrane; lysosomal membrane; plasma membrane; trans-Golgi network membrane

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; T cell costimulation; T cell receptor signaling pathway