CRYBB2 sirna

CRYBB2 siRNA (Mouse)

Cat. Num. AAA8221273

• Gene Names: Crybb2; Cryb-2
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CRYBB2; CRYBB2 siRNA (Mouse); Beta-crystallin B2; Beta-B2 crystallin; Beta-crystallin Bp; CRYBB2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CRYBB2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 205

• Applicable Applications for CRYBB2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CRYBB2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CRYBB2 sirna

siRNA to inhibit CRYBB2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6681035
• NCBI GeneID: 12961
• NCBI Accession #: NP_031799.1
• NCBI GenBank Nucleotide #: NM_007773.4
• UniProt Accession #: P62696; P19942; P26775; Q0VGT6
• Molecular Weight: 23,381 Da
• NCBI Official Full Name: beta-crystallin B2
• NCBI Official Synonym Full Names: crystallin, beta B2
• NCBI Official Symbol: Crybb2
• NCBI Official Synonym Symbols: Cryb-2
• NCBI Protein Information: beta-crystallin B2
• UniProt Protein Name: Beta-crystallin B2
• Protein Family: Beta-crystallin
• UniProt Gene Name: Crybb2
• UniProt Entry Name: CRBB2_MOUSE

NCBI Description

This gene is a member of the beta-crystallin family. Beta crystallins, along with alpha and gamma crystallins, are the major proteins found in the eye lens. These proteins maintain the refractive index of the lens whilst also maintaining its transparency. Since lens central fiber cells lose their nuclei during development, crystallins are made and then retained throughout life, making them extremely stable proteins. Beta and gamma crystallins are considered be a superfamily and have a similar domain architecture, including four Greek Key motifs. Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. The protein encoded by this gene may have Ca2+-binding activity and could be associated with potential functions in the hippocampus and in sperm. Targeted knockout of this gene in mouse induces age-related cataract. A chain-terminating mutation in a similar gene in human was found to cause type 2 cerulean cataracts. [provided by RefSeq, Feb 2015]

Uniprot Description

CRYBB2: a major structural protein of the eye lens. A member of the beta/gamma-crystallin family. Defects are the cause of congenital cerulean type 2 cataract, sutural cataract with punctate and cerulean opacities, and Coppock-like cataract.

Molecular Function: identical protein binding; protein homodimerization activity; structural constituent of eye lens

Biological Process: camera-type eye development; visual perception