Threonine Aspartase 1 Recombinant Protein | TASP1 recombinant protein

Recombinant Threonine Aspartase 1 (TASP1)

Cat. Num. AAA2032937

• Gene Names: TASP1; C20orf13; dJ585I14.2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Threonine Aspartase 1; Recombinant Threonine Aspartase 1 (TASP1); Taspase-1; C20orf13; TASP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: TVGAVVV DHEGNVAAAV SSGGLALKHP GRVGQAALYG CGCWAENTGA HNPYSTAVST SGCGEHLVRT ILARECSHAL QAEDAHQALL ETMQNKFISS PFLASEDGVL GGVIVLRSCR CSAEPDSSQN KQTLLVEFLW SHTTESMCVG YMSAQDGKAK THISRLPPGA VAGQSVAIEGGVCRLESPVN
• Sequence Length: 420

• Applicable Applications for TASP1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 23.1kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Thr234~Asn420
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 98991769
• NCBI GeneID: 55617
• NCBI Accession #: NP_060184.2
• NCBI GenBank Nucleotide #: NM_017714.2
• Molecular Weight: 15,946 Da
• NCBI Official Full Name: threonine aspartase 1
• NCBI Official Synonym Full Names: taspase 1
• NCBI Official Symbol: TASP1
• NCBI Official Synonym Symbols: C20orf13; dJ585I14.2
• NCBI Protein Information: threonine aspartase 1
• UniProt Protein Name: Threonine aspartase 1
• Protein Family: Threonine aspartase
• UniProt Gene Name: TASP1
• UniProt Synonym Gene Names: C20orf13; Taspase-1
• UniProt Entry Name: TASP1_HUMAN

NCBI Description

This gene encodes an endopeptidase that cleaves specific substrates following aspartate residues. The encoded protein undergoes posttranslational autoproteolytic processing to generate alpha and beta subunits, which reassemble into the active alpha2-beta2 heterotetramer. It is required to cleave MLL, a protein required for the maintenance of HOX gene expression, and TFIIA, a basal transcription factor. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TASP1: Protease involved in MLL processing and, consequently, in the correct expression of the early HOXA gene cluster. Belongs to the Ntn-hydrolase family.

Protein type: EC 3.4.25.-; Protease

Chromosomal Location of Human Ortholog: 20p12.1

Molecular Function: threonine endopeptidase activity

Biological Process: positive regulation of transcription, DNA-dependent; proteolysis