Rabbit anti-Human, Mouse RRAGD Polyclonal Antibody | anti-RRAGD antibody

RRAGD, ID (RRAGD, Ras-related GTP-binding protein D) (Biotin)

Cat. Num. AAA6344053

• Gene Names: RRAGD; RAGD; bA11D8.2.1
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RRAGD; Polyclonal Antibody; ID (RRAGD; Ras-related GTP-binding protein D) (Biotin); Ras-related GTP-binding protein D; anti-RRAGD antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RRAGD antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RRAGD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 180-210 from the Central region of human RRAGD.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RRAGD Antibody (Center) in MDA-MB435 cell line lysates (35ug/lane). RRAGD (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of RRAGD Antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). RRAGD (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(RRAGD Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RRAGD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RRAGD Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RRAGD antibody

RRAGD is a monomeric guanine nucleotide-binding protein, or G protein. By binding GTP or GDP, small G proteins act as molecular switches in numerous cell processes and signaling pathways.

Product Categories/Family for anti-RRAGD antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Olsen, J.V., et al. Cell 127(3):635-648(2006). de Wit, N.J., et al. Br. J. Cancer 92(12):2249-2261(2005). Sekiguchi, T., et al. J. Biol. Chem. 279(9):8343-8350(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 11034847
• NCBI GeneID: 58528
• NCBI Accession #: NP_067067.1
• NCBI GenBank Nucleotide #: NM_021244.4
• UniProt Accession #: Q9NQL2; Q7L8F9; Q9NPG0; A8K184
• Molecular Weight: 45,588 Da
• NCBI Official Full Name: ras-related GTP-binding protein D
• NCBI Official Synonym Full Names: Ras-related GTP binding D
• NCBI Official Symbol: RRAGD
• NCBI Official Synonym Symbols: RAGD; bA11D8.2.1
• NCBI Protein Information: ras-related GTP-binding protein D; Rag D protein
• UniProt Protein Name: Ras-related GTP-binding protein D
• Protein Family: Ras-related GTP-binding protein
• UniProt Gene Name: RRAGD
• UniProt Synonym Gene Names: Rag D; RagD
• UniProt Entry Name: RRAGD_HUMAN

NCBI Description

RRAGD is a monomeric guanine nucleotide-binding protein, or G protein. By binding GTP or GDP, small G proteins act as molecular switches in numerous cell processes and signaling pathways.[supplied by OMIM, Apr 2004]

Uniprot Description

RRAGD: Has guanine nucleotide-binding activity but lacks intrinsic GTPase activity. Probably required for the amino acid- induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Forms a heterodimer with RRAGA in a sequence-independent manner. Binds GTP. Interacts with NOL8 and RRAGB. Belongs to the GTR/RAG GTP-binding protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein regulator, misc.

Chromosomal Location of Human Ortholog: 6q15

Cellular Component: lysosome; cytoplasm; nucleus

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; protein heterodimerization activity

Biological Process: metabolic process; regulation of autophagy; cellular response to starvation; positive regulation of TOR signaling pathway